Recombinant Anti-NRK1 antibody [EPR11190] (ab169548)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11190] to NRK1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-NRK1 antibody [EPR11190]
See all NRK1 primary antibodies -
Description
Rabbit monoclonal [EPR11190] to NRK1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human NRK1.
Database link: Q9NWW6 -
Positive control
- IM-9, LoVo, HT-29 and MCF7 cell lysates; HT-29 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11190 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 23 kDa.
|
Notes |
---|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 23 kDa. |
Target
-
Function
Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). The enzyme also phosphorylates the antitumor drugs tiazofurin and 3-deazaguanosine. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis. -
Sequence similarities
Belongs to the uridine kinase family. NRK subfamily. - Information by UniProt
-
Database links
- Entrez Gene: 54981 Human
- Entrez Gene: 499330 Rat
- Omim: 608704 Human
- SwissProt: Q9NWW6 Human
- SwissProt: Q6AY91 Rat
- Unigene: 494186 Human
- Unigene: 19716 Rat
-
Alternative names
- bA235O14.2 antibody
- C9orf95 antibody
- Chromosome 9 open reading frame 95 antibody
see all
Images
-
All lanes : Anti-NRK1 antibody [EPR11190] (ab169548) at 1/10000 dilution
Lane 1 : IM-9 cell lysate
Lane 2 : LoVo cell lysate
Lane 3 : HT-29 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa -
Intracellular flow cytometric analysis of permeabilized HT-29 cells labeling NRK1 with ab169548 at 1/10 dilution (red) compared a rabbit IgG negative control (green).
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab169548 has been referenced in 2 publications.
- Wilk A et al. Extracellular NAD+ enhances PARP-dependent DNA repair capacity independently of CD73 activity. Sci Rep 10:651 (2020). PubMed: 31959836
- Chowdhry S et al. NAD metabolic dependency in cancer is shaped by gene amplification and enhancer remodelling. Nature 569:570-575 (2019). PubMed: 31019297