Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11190] to NRK1
- Suitable for: WB, Flow Cyt
- Reacts with: Rat, Human
Product nameAnti-NRK1 antibody [EPR11190]
See all NRK1 primary antibodies
DescriptionRabbit monoclonal [EPR11190] to NRK1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to Human NRK1.
Database link: Q9NWW6
- IM-9, LoVo, HT-29 and MCF7 cell lysates; HT-29 cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Previously labelled as C9orf95.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 23 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). The enzyme also phosphorylates the antitumor drugs tiazofurin and 3-deazaguanosine.
PathwayCofactor biosynthesis; NAD(+) biosynthesis.
Sequence similaritiesBelongs to the uridine kinase family. NRK subfamily.
- Information by UniProt
- bA235O14.2 antibody
- C9orf95 antibody
- Chromosome 9 open reading frame 95 antibody
All lanes : Anti-NRK1 antibody [EPR11190] (ab169548) at 1/10000 dilution
Lane 1 : IM-9 cell lysate
Lane 2 : LoVo cell lysate
Lane 3 : HT-29 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Flow cytometric analysis of permeabilized HT-29 cells labeling NRK1 with ab169548 at 1/10 dilution (red) compared a rabbit IgG negative control (green).
ab169548 has been referenced in 1 publication.
- Wilk A et al. Extracellular NAD+ enhances PARP-dependent DNA repair capacity independently of CD73 activity. Sci Rep 10:651 (2020). PubMed: 31959836