Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NRXN3 antibody

Poor
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain

Other product details

Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
I tried this antibody at different dilutions. The only staining I observed was observed on the walls of blood vessels. This pattern was observed in all brain areas. The pictures shows the staining observed at the level of the thalamus.

The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Abcam response
Thank you for your valuable feedback. Ab18523 is a Fast Track antibody and has not yet been fully characterized. it appears from your data that the antibody may not be suitable for IHC-FoFr under these experimental conditions. We hope this information is useful to our customers.

Dr. Sophie Pezet

Verified customer

Submitted May 09 2011

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