NS 398, COX-2 inhibitor (ab120295)
Key features and details
- COX-2 inhibitor
- CAS Number: 123653-11-2
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
NS 398, COX-2 inhibitor -
Description
COX-2 inhibitor -
Biological description
Selective cyclooxygenase-2 inhibitor (IC50 values are 3.8 and >100 μM for COX-2 and COX-1, respectively). Orally active. Anti-inflammatory, antipyretic, analgesic and non-ulcerogenic in vivo. Induces apoptosis and cell cycle arrest.
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CAS Number
123653-11-2 -
Chemical structure
Properties
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Chemical name
N-[2-(Cyclohexyloxy)-4-nitrophenyl]methanesulfonamide -
Molecular weight
314.36 -
Molecular formula
C13H18N2O5S -
PubChem identifier
4553 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[O-][N+](=O)c2cc(OC1CCCCC1)c(NS(C)(=O)=O)cc2 -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - NS 398, COX-2 inhibitor (ab120295)2D chemical structure image of ab120295, NS 398, COX-2 inhibitor
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Functional Studies - NS 398, COX-2 inhibitor (ab120295)ab32034 staining p27 KIP1 in MCF7 cells treated with NS 398 (ab120295), by ICC/IF. Increase in p27 KIP1 expression correlates with increased concentration of NS 398, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120295 (NS 398) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32034 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (3)
ab120295 has been referenced in 3 publications.
- Bagayoko S et al. Host phospholipid peroxidation fuels ExoU-dependent cell necrosis and supports Pseudomonas aeruginosa-driven pathology. PLoS Pathog 17:e1009927 (2021). PubMed: 34516571
- Cong J et al. Astroglial CB1 Cannabinoid Receptors Mediate CP 55,940-Induced Conditioned Place Aversion Through Cyclooxygenase-2 Signaling in Mice. Front Cell Neurosci 15:772549 (2021). PubMed: 34887729
- Ishihara E et al. Prostaglandin E2 and its receptor EP2 trigger signaling that contributes to YAP-mediated cell competition. Genes Cells 25:197-214 (2020). PubMed: 31989743