NS 398, COX-2 inhibitor (ab120295)

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Chemical Structure - NS 398, COX-2 inhibitor (ab120295)
  • Functional Studies - NS 398, COX-2 inhibitor (ab120295)

Key features and details

  • COX-2 inhibitor
  • CAS Number: 123653-11-2
  • Purity: > 98%
  • Soluble in DMSO to 100 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    NS 398, COX-2 inhibitor

  • Description

    COX-2 inhibitor

  • Biological description

    Selective cyclooxygenase-2 inhibitor (IC50 values are 3.8 and >100 μM for COX-2 and COX-1, respectively). Orally active. Anti-inflammatory, antipyretic, analgesic and non-ulcerogenic in vivo. Induces apoptosis and cell cycle arrest.

  • Purity

    > 98%

  • CAS Number

    123653-11-2

  • Chemical structure

    Chemical Structure

Properties

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab120295 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies
    Use at an assay dependent concentration.
    Notes
    Functional Studies
    Use at an assay dependent concentration.

    Images

    • Chemical Structure - NS 398, COX-2 inhibitor (ab120295)
      Chemical Structure - NS 398, COX-2 inhibitor (ab120295)
      2D chemical structure image of ab120295, NS 398, COX-2 inhibitor
    • Functional Studies - NS 398, COX-2 inhibitor (ab120295)
      Functional Studies - NS 398, COX-2 inhibitor (ab120295)
      ab32034 staining p27 KIP1 in MCF7 cells treated with NS 398 (ab120295), by ICC/IF. Increase in p27 KIP1 expression correlates with increased concentration of NS 398, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120295 (NS 398) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32034 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab120295? Please let us know so that we can cite the reference in this datasheet.

    ab120295 has been referenced in 1 publication.

    • Ishihara E  et al. Prostaglandin E2 and its receptor EP2 trigger signaling that contributes to YAP-mediated cell competition. Genes Cells 25:197-214 (2020). PubMed: 31989743

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Thanks. I am also currently looking for a VEGF inhibitor and mostly they are for the receptor. Any chemical inhibitor in line with NS-398 should work fine for us. May be if I get one, I will let you know.

    Best Regards,

    Read More
    Answer

    Thank you for your reply.

    That is the problem with this target. I have been able to find chemical inhibitors of VEGFR but not for VEGF. There seems to be mainly antibody inhibitors of VEGF. If you do find a compound you are interested in, please do let me know and I can see if we could consider adding it to the catalogue.

    I am sorry I could not be of more help in this instance. If you have any further questions, please do not hesitate to contact us again.

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    Question

    I am looking for primary HO-1 antibody (Haem oxygnase) and COX-2 (mono or polyclonal , raised in rabbit against mouse, rat and human, applicability). Can you suggest some good ones. Also, I am looking for chemical inhibitors for COX-2 (NS-398) and VEGF (I actually don’t know the name of the inhibitor), can you tell me the availability of the same?

    Best Regards,

    Read More
    Answer

    I hope your grant application is going well. We have several antibodies against the targets you have mentioned which may be of interest to you:

    HO-1

    I would suggest looking at the rabbit polyclonal https://www.abcam.com/Heme-Oxygenase-1-antibody-ab13243.html. This has been shown to react with human, mouse and rat samples and has been used in ICC, IHC-Paraffin embedded, sELISA and Western blotting to date. We also have a very popular mouse monoclonal https://www.abcam.com/Heme-Oxygenase-1-antibody-HO-1-1-ab13248.htmlwhich has also been used with human, mouse and rat samples as well as in ELISA, Flow Cytometry, ICC,IHC-Frozen sections, and Western blotting. Bit I think you were more interested in antibodies raised in rabbit.

    COX-2

    For this target I would suggest having a look at the rabbit polyclonal https://www.abcam.com/COX2--Cyclooxygenase-2-antibody-ab15191.html. This has again been tested with mouse, rat and human samples and has been used in ICC/IF, IHC-Frozen sections, IHC-Parrafin embedded, IP, sELISA and Westernblotting.

    We also have the NS-398 compound (COX-2 inhibitor) which is available under the catalogue number https://www.abcam.com/NS-398-ab120295.html.

    I am afraid I have not been able to find a similar inhibitor of VEGF. Do you have a particular compound in mind? It may be that I can suggest we start stocking it.

    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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    Question

    Thank you very much for your prompt reply. If possible can you advice me as to which product is the best. I require a COX2 inhibitor to put onto cells ?



    Many Thanks

    Read More
    Answer

    Thank you for your reply.

    I am happy to confirm that we have a COX2 inhibitor in our catalog.

    ab120295 (NS 398)

    https://www.abcam.com/NS-398-COX-2-inhibitor-ab120295.html (or use the following: https://www.abcam.com/NS-398-COX-2-inhibitor-ab120295.html).

    This product was used in three publications we know of. Please review the publication for more information on the use of ab120295 in vitro:

    Du Y et al. Induction of apoptosis and cell cycle arrest by NS398 in oral squamous cell carcinoma cells via downregulation of E2 promoter-binding factor-1. Oncol Rep 20:605-11 (2008). PubMed: 18695912

    Futaki N et al. NS-398, a new anti-inflammatory agent, selectively inhibits prostaglandin G/H synthase/cyclooxygenase (COX-2) activity in vitro. Prostaglandins 47:55-9 (1994). PubMed: 8140262

    Futaki N et al. NS-398, a novel non-steroidal anti-inflammatory drug with potent analgesic and antipyretic effects, which causes minimal stomach lesions. Gen Pharmacol 24:105-10 (1993). PubMed: 8482483

    I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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