NS 398, COX-2 inhibitor (ab120295)

Submit a review Q&A (3)

Overview

  • Product name
    NS 398, COX-2 inhibitor
  • Description
    COX-2 inhibitor
  • Biological description
    Selective cyclooxygenase-2 inhibitor (IC50 values are 3.8 and >100 μM for COX-2 and COX-1, respectively). Orally active. Anti-inflammatory, antipyretic, analgesic and non-ulcerogenic in vivo. Induces apoptosis and cell cycle arrest.
  • Purity
    > 98%
  • CAS Number
    123653-11-2
  • Chemical structure
    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Functional Studies - NS 398, COX-2 inhibitor (ab120295)
    Functional Studies - NS 398, COX-2 inhibitor (ab120295)
    ab32034 staining p27 KIP1 in MCF7 cells treated with NS 398 (ab120295), by ICC/IF. Increase in p27 KIP1 expression correlates with increased concentration of NS 398, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120295 (NS 398) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32034 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

References

This product has been referenced in:
  • Du Y  et al. Induction of apoptosis and cell cycle arrest by NS398 in oral squamous cell carcinoma cells via downregulation of E2 promoter-binding factor-1. Oncol Rep 20:605-11 (2008). Read more (PubMed: 18695912) »
  • Futaki N  et al. NS-398, a new anti-inflammatory agent, selectively inhibits prostaglandin G/H synthase/cyclooxygenase (COX-2) activity in vitro. Prostaglandins 47:55-9 (1994). Read more (PubMed: 8140262) »
  • Futaki N  et al. NS-398, a novel non-steroidal anti-inflammatory drug with potent analgesic and antipyretic effects, which causes minimal stomach lesions. Gen Pharmacol 24:105-10 (1993). Read more (PubMed: 8482483) »

Publishing research using ab120295? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Thanks. I am also currently looking for a VEGF inhibitor and mostly they are for the receptor. Any chemical inhibitor in line with NS-398 should work fine for us. May be if I get one, I will let you know.

Best Regards,

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Answer

Thank you for your reply.

That is the problem with this target. I have been able to find chemical inhibitors of VEGFR but not for VEGF. There seems to be mainly antibody inhibitors of VEGF. If you do find a compound you are interested in, please do let me know and I can see if we could consider adding it to the catalogue.

I am sorry I could not be of more help in this instance. If you have any further questions, please do not hesitate to contact us again.

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Question

I am looking for primary HO-1 antibody (Haem oxygnase) and COX-2 (mono or polyclonal , raised in rabbit against mouse, rat and human, applicability). Can you suggest some good ones. Also, I am looking for chemical inhibitors for COX-2 (NS-398) and VEGF (I actually don’t know the name of the inhibitor), can you tell me the availability of the same?

Best Regards,

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Answer

I hope your grant application is going well. We have several antibodies against the targets you have mentioned which may be of interest to you:

HO-1

I would suggest looking at the rabbit polyclonal https://www.abcam.com/Heme-Oxygenase-1-antibody-ab13243.html. This has been shown to react with human, mouse and rat samples and has been used in ICC, IHC-Paraffin embedded, sELISA and Western blotting to date. We also have a very popular mouse monoclonal https://www.abcam.com/Heme-Oxygenase-1-antibody-HO-1-1-ab13248.htmlwhich has also been used with human, mouse and rat samples as well as in ELISA, Flow Cytometry, ICC,IHC-Frozen sections, and Western blotting. Bit I think you were more interested in antibodies raised in rabbit.

COX-2

For this target I would suggest having a look at the rabbit polyclonal https://www.abcam.com/COX2--Cyclooxygenase-2-antibody-ab15191.html. This has again been tested with mouse, rat and human samples and has been used in ICC/IF, IHC-Frozen sections, IHC-Parrafin embedded, IP, sELISA and Westernblotting.

We also have the NS-398 compound (COX-2 inhibitor) which is available under the catalogue number https://www.abcam.com/NS-398-ab120295.html.

I am afraid I have not been able to find a similar inhibitor of VEGF. Do you have a particular compound in mind? It may be that I can suggest we start stocking it.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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Question

Thank you very much for your prompt reply. If possible can you advice me as to which product is the best. I require a COX2 inhibitor to put onto cells ?



Many Thanks

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Answer

Thank you for your reply.

I am happy to confirm that we have a COX2 inhibitor in our catalog.

ab120295 (NS 398)

https://www.abcam.com/index.html?datasheet=120295 (or use the following: https://www.abcam.com/index.html?datasheet=120295).

This product was used in three publications we know of. Please review the publication for more information on the use of ab120295 in vitro:

Du Y et al. Induction of apoptosis and cell cycle arrest by NS398 in oral squamous cell carcinoma cells via downregulation of E2 promoter-binding factor-1. Oncol Rep 20:605-11 (2008). PubMed: 18695912

Futaki N et al. NS-398, a new anti-inflammatory agent, selectively inhibits prostaglandin G/H synthase/cyclooxygenase (COX-2) activity in vitro. Prostaglandins 47:55-9 (1994). PubMed: 8140262

Futaki N et al. NS-398, a novel non-steroidal anti-inflammatory drug with potent analgesic and antipyretic effects, which causes minimal stomach lesions. Gen Pharmacol 24:105-10 (1993). PubMed: 8482483

I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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