Product nameNSC 146109 hydrochloride, Cell-permeable p53 activator
DescriptionCell-permeable p53 activator
Biological descriptionCell-permeable p53 activator. Genotype-selective antitumor agent. Active in vitro.
Chemical name(10-Methylanthracen-9-yl)methyl carbamimidothioate hydrochloride
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
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Our Abpromise guarantee covers the use of ab142144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab26 staining p53 in U20S cells treated with NSC 146109 hydrochloride (ab142144), by ICC/IF. Increase in p53 expression correlates with increased concentration of NSC 146109 hydrochloride, as described in literature.
The cells were incubated at 37°C for 5 hour in media containing different concentrations of ab142144 (NSC 146109 hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab26 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Wang H & Yan C A small-molecule p53 activator induces apoptosis through inhibiting MDMX expression in breast cancer cells. Neoplasia 13:611-9 (2011). Read more (PubMed: 21750655) »
- Berkson RG et al. Pilot screening programme for small molecule activators of p53. Int J Cancer 115:701-10 (2005). Read more (PubMed: 15729694) »
- Dolma S et al. Identification of genotype-selective antitumor agents using synthetic lethal chemical screening in engineered human tumor cells. Cancer Cell 3:285-96 (2003). Read more (PubMed: 12676586) »