NSC 146109 hydrochloride, Cell-permeable p53 activator (ab142144)

Overview

  • Product name

    NSC 146109 hydrochloride, Cell-permeable p53 activator
  • Description

    Cell-permeable p53 activator
  • Biological description

    Cell-permeable p53 activator. Genotype-selective antitumor agent. Active in vitro.
  • Purity

    > 98%
  • CAS Number

    59474-01-0
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    (10-Methylanthracen-9-yl)methyl carbamimidothioate hydrochloride
  • Molecular weight

    316.85
  • Molecular formula

    C17H16N2S.HCl
  • PubChem identifier

    16759161
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 100 mM and in ethanol to 10 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC1=C2C=CC=CC2=C(C3=CC=CC=C13)CSC(=N)N.Cl
  • Source

    Synthetic

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab142144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab26 staining p53 in U20S cells treated with NSC 146109 hydrochloride (ab142144), by ICC/IF. Increase in p53 expression correlates with increased concentration of NSC 146109 hydrochloride, as described in literature.
    The cells were incubated at 37°C for 5 hour in media containing different concentrations of ab142144 (NSC 146109 hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab26 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:

  • Wang H & Yan C A small-molecule p53 activator induces apoptosis through inhibiting MDMX expression in breast cancer cells. Neoplasia 13:611-9 (2011). Read more (PubMed: 21750655) »
  • Berkson RG  et al. Pilot screening programme for small molecule activators of p53. Int J Cancer 115:701-10 (2005). Read more (PubMed: 15729694) »
  • Dolma S  et al. Identification of genotype-selective antitumor agents using synthetic lethal chemical screening in engineered human tumor cells. Cancer Cell 3:285-96 (2003). Read more (PubMed: 12676586) »

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab142144.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

Sign up