Key features and details
- Rabbit polyclonal to NSD3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NSD3 antibody
See all NSD3 primary antibodies
DescriptionRabbit polyclonal to NSD3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment, corresponding to a region within internal sequence amino acids 821-1062 of Human NSD3.
- 293T, HeLa, Raji and Mouse brain cell lysates; HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 162 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionHistone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
Tissue specificityHighly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
Involvement in diseaseDefects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.
A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.
Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
Contains 1 AWS domain.
Contains 4 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 2 PWWP domains.
Contains 1 SET domain.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- DKFZp667H044 antibody
- FLJ20353 antibody
- Histone lysine N methyltransferase NSD3 antibody
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling NSD3 with ab137430 at 1/200 dilution. Lower panel merged with DNA probe.
Anti-NSD3 antibody (ab137430) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 162 kDa
Anti-NSD3 antibody (ab137430) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 162 kDa
5% SDS PAGE.
ab137430 has been referenced in 1 publication.
- Wang C et al. The methyltransferase NSD3 promotes antiviral innate immunity via direct lysine methylation of IRF3. J Exp Med 214:3597-3610 (2017). PubMed: 29101251