• Product name
  • Description
    Rabbit polyclonal to NSD3
  • Host species
  • Specificity
    This antibody should recognise both the 72 and 161 kDa isoforms: NP_060248 (645 aa) - 72.6 kDa, NP_075447 (1437 aa) - 161 kDa.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from residues 500 - 600 of Human NSD3.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, HEK293.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Breast Carcinoma.

      This antibody gave a positive signal in the following cell lines: HeLa.



Our Abpromise guarantee covers the use of ab4514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72.6, 161 kDa (predicted molecular weight: 72.6 , 161 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.


  • Function
    Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
  • Tissue specificity
    Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
  • Involvement in disease
    Defects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.
    A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.
  • Sequence similarities
    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 4 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 2 PWWP domains.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp667H044 antibody
    • FLJ20353 antibody
    • Histone lysine N methyltransferase NSD3 antibody
    • Histone-lysine N-methyltransferase NSD3 antibody
    • MGC126766 antibody
    • MGC142029 antibody
    • NSD 3 antibody
    • NSD3_HUMAN antibody
    • Nuclear set domain containing 3 antibody
    • Nuclear SET domain containing protein 3 antibody
    • Nuclear SET domain-containing protein 3 antibody
    • pp14328 antibody
    • Protein whistle antibody
    • Whistle antibody
    • WHSC1 like protein 1 antibody
    • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody
    • WHSC1-like protein 1 antibody
    • WHSC1L1 antibody
    • WHSC1L1 protein antibody
    • Wolf Hirschhorn syndrome candidate 1 like 1 antibody
    • Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody
    see all


  • All lanes : Anti-NSD3 antibody (ab4514) at 1 µg/ml

    Lane 1 : HeLa whole cell extract
    Lane 2 : HEK293 cell extract
    Lane 3 : HeLa whole cell extract with Human NSD3 peptide (ab14995) at 1 µg/ml
    Lane 4 : HEK293 cell extract with Human NSD3 peptide (ab14995) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 72.6 , 161 kDa
    Observed band size: 161,72.6 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 115 kDa (possible cross reactivity)

    ab4514 specifically recognises NSD3 (lane1) in HeLa whole cell and HEK293 (lane3) cell extracts at 72.6 kDa and 161 kDa, which correspond to two different isoforms of NSD3. The signal can be quenched using the immunising peptide (lanes3-4) indicating that ab4514 specifically recognises NSD3.
  • Breast carcinoma tissue was fixed in 10% formalin at 4°C overnight and paraffin embedded. Following blocking the tissue sections were incubated with ab4514 (1/50 dilution) at 37°C for 1 hour, and with the secondary antibody at 37°C for 30 minutes. The slides were counterstained with Hematoxylin. Washes were carried out using TBS. Heat mediated antigen retrieval was carried out using the Microwave method before commencing the staining protocol. ab4514 staining appears to be localized mainly to the cytoplasm. Although the putative cellular localization of NSD3 is the nucleus we have found no evidence in the current literature to confirm this and we believe ab4514 may be detecting a cytoplasmic form of the protein. We would be very grateful for any customer feedback about NSD3 and ab4514.

  • ICC/IF image of ab4514 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4514, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    As seen in Immunohistochemical analysis (shown above), although the putative cellular localization of NSD3 is the nucleus we have found no evidence in the current literature to confirm this and we believe ab4514 may be detecting a cytoplasmic form of the protein.


This product has been referenced in:
  • Rahman S  et al. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol 31:2641-52 (2011). Read more (PubMed: 21555454) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Immuno-precipitation step
Protein A
Human Cell lysate - nuclear (Hela)
Total protein in input
4e+007 cells

Abcam user community

Verified customer

Submitted Jun 03 2013


Thank you for confirming this with customer.

I have contacted the laboratory for checking if they are happy to send a free vial to this customer. I will contact you soon with more updates.

Though I will be able to sort this case this week however if you will want any recent updates please do not hesitate ate to contact me.

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Thank you for contacting us.

Unfortunately we have no stock available at the moment. We do have an lot in characterisation which has passed but our WB band is running higher than expected. Our scientists are working with collaborators to find out more information about this ab and whether this higher band is the protein of interest.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you very much for your call yesterday and for letting us know about the trouble with ab4514.

As we discussed, I'm sending a free of charge vial of the HeLa cell lysate, ab29545, on the order *** which should arrive today.

Please keep me updated about the results with this lysate, and let me know if there is anything else that we can do for you.

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Thank you for getting back to me with further information. I have passed on the details to my business development colleagues and we will discuss the possibility of starting an immunisation program against this target. We are always keen to get our antibodies tested in ChIP and available to our customers as "ChIP grade". We regularly send out marketing e-mails informing our customers of new additions to our ChIP grade antibodies. I am pleased to hear that you are interested in our NSD1 antibody in production. Once again thank you for the details and I look forward to meeting you at one of our regular chromatin meetings.

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