Recombinant
RabMAb

Recombinant Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)

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Overview

  • Product name

    Anti-NSD3 antibody [EPR13813] - N-terminal
    See all NSD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13813] to NSD3 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NSD3 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9BZ95

  • Positive control

    • Hela, A673 and MCF7 lysates; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab180500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 162 kDa.
Flow Cyt 1/70.

For unpurified, use 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.

    • Tissue specificity

      Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.

    • Involvement in disease

      Defects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.
      A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.

    • Sequence similarities

      Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
      Contains 1 AWS domain.
      Contains 4 PHD-type zinc fingers.
      Contains 1 post-SET domain.
      Contains 2 PWWP domains.
      Contains 1 SET domain.

    • Cellular localization

      Nucleus. Chromosome.
    • Information by UniProt

    • Database links

      • Alternative names

        • DKFZp667H044 antibody
        • FLJ20353 antibody
        • Histone lysine N methyltransferase NSD3 antibody
        • Histone-lysine N-methyltransferase NSD3 antibody
        • MGC126766 antibody
        • MGC142029 antibody
        • NSD 3 antibody
        • NSD3_HUMAN antibody
        • Nuclear set domain containing 3 antibody
        • Nuclear SET domain containing protein 3 antibody
        • Nuclear SET domain-containing protein 3 antibody
        • pp14328 antibody
        • Protein whistle antibody
        • Whistle antibody
        • WHSC1 like protein 1 antibody
        • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody
        • WHSC1-like protein 1 antibody
        • WHSC1L1 antibody
        • WHSC1L1 protein antibody
        • Wolf Hirschhorn syndrome candidate 1 like 1 antibody
        • Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody
        see all

      Images

      • Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (unpurified)

        Lane 1 : Hela lysate
        Lane 2 : A673 lysate
        Lane 3 : MCF7 lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Predicted band size: 162 kDa

      • Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)

        Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab180500 at a dilution of 1 in 70 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of primary and secondary antibody were used as a negative control (blue line).

      • Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified) + Rat kidney tissue lysate at 20 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 162 kDa
        Observed band size: 162 kDa



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/10000 dilution (purified)

        Lane 1 : Mouse spleen tissue lysate
        Lane 2 : Rat spleen tissue lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 162 kDa
        Observed band size: 162 kDa



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified)

        Lane 1 : HeLa cell lysate
        Lane 2 : MCF7 cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 162 kDa
        Observed band size: 162 kDa



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)
        Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)

        Flow Cytometrical analysis of HeLa cells labeling NSD3 with unpurified ab180500 at 1/75 (green) compared to a negative control antibody (red).

      References

      This product has been referenced in:

      • Papillon-Cavanagh S  et al. Impaired H3K36 methylation defines a subset of head and neck squamous cell carcinomas. Nat Genet 49:180-185 (2017). Read more (PubMed: 28067913) »
      • Cheng C  et al. Whole-Genome Sequencing Reveals Diverse Models of Structural Variations in Esophageal Squamous Cell Carcinoma. Am J Hum Genet 98:256-74 (2016). WB . Read more (PubMed: 26833333) »
      See all 2 Publications for this product

      Publishing research using ab180500? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-NSD3 [EPR13813] antibody - N-terminal

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (10 % GEL, SDS)
      Sample
      Human Cell lysate - whole cell (HEK293, HELA, HCT116)
      Specification
      HEK293, HELA, HCT116
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 18 2014

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