Overview

  • Product name

    Anti-NSDHL antibody [EPR14489(2)]
    See all NSDHL primary antibodies
  • Description

    Rabbit monoclonal [EPR14489(2)] to NSDHL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NSDHL aa 100-200. The exact sequence is proprietary.
    Database link: Q15738

  • Positive control

    • WB: A431, HeLa, HepG2, NIH/3T3, PC-12 and C6 whole cell lysates; Human fetal brain lysate. IP: Human fetal brain whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IP 1/50.

Target

  • Tissue specificity

    Brain, heart, liver, lung, kidney, skin and placenta.
  • Pathway

    Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.
  • Involvement in disease

    Defects in NSDHL are the cause of congenital hemidysplasia with ichthyosiform erythroderma and limb defects (CHILD) [MIM:308050]. CHILD is an X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, which typically results in male lethality. Clinically, it is characterized by congenital, unilateral, ichthyosisform erythroderma with striking lateralization, sharp midline demarcation, and ipsilateral limb defects and hypoplasia of the body. Limbs defects range from hypoplasia of digits or ribs to complete amelia, often including scoliosis.
    Defects in NSDHL are the cause of CK syndrome (CKS) [MIM:300831]. CKS is a disorder characterized by mild to severe cognitive impairment, seizures, microcephaly, cerebral cortical malformations, dysmorphic facial features, and thin body habitus.
  • Sequence similarities

    Belongs to the 3-beta-HSD family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • decarboxylating antibody
    • H105E3 antibody
    • H105e3 protein antibody
    • NAD(P) dependent steroid dehydrogenase like antibody
    • NSDHL antibody
    • NSDHL_HUMAN antibody
    • Protein H105e3 antibody
    • SDR31E1 antibody
    • Short chain dehydrogenase/reductase family 31E member 1 antibody
    • Sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating antibody
    • Sterol-4-alpha-carboxylate 3-dehydrogenase antibody
    • XAP104 antibody
    see all

Images

  • All lanes : Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilution

    Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated) at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilution

    Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : C6 (Rat glial tumor cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 10 seconds


    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% NFDM /TBST or 1%BSA /TBST.

  • NSDHL was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab199730 at 1/50 dilution.

    Lane 1: Human fetal brain whole cell lysate 10ug (Input).

    Lane 2: ab199730 IP in Human fetal brain whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199730 in Human fetal brain whole cell lysate.

    Western blot was performed from the immunoprecipitate using ab199730 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • NSDHL was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab199730 at 1/50 dilution.

    Lane 1: HeLa whole cell lysate 10ug (Input).

    Lane 2: ab199730 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199730 in HeLa whole cell lysate.

    Western blot was performed from the immunoprecipitate using ab199730 at 1/1000 dilution (Panel A) or ab190353 at 1/1000 dilution (Panel B).

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds (Panel A and B).

     ab199730 was used to immunoprecipitate NSDHL. These precipitates were resolved with ab190353.

References

ab199730 has not yet been referenced specifically in any publications.

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