Recombinant Anti-NSDHL antibody [EPR14490] (ab190353)
![Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)](https://www.abcam.com/ps/products/190/ab190353/Images/ab190353-410148-anti-nsdhl-antibody-epr14490-western-blot-human-knockout-lysate.jpg "Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14490] to NSDHL
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NSDHL antibody [EPR14490]
See all NSDHL primary antibodies -
Description
Rabbit monoclonal [EPR14490] to NSDHL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, A431, HeLa and Human fetal brain lysates. IHC-P: Human liver and Human adenocarcinoma of endometrium tissue. ICC: U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14490 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 42 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/50.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 42 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/50. |
Target
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Tissue specificity
Brain, heart, liver, lung, kidney, skin and placenta. -
Pathway
Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6. -
Involvement in disease
Defects in NSDHL are the cause of congenital hemidysplasia with ichthyosiform erythroderma and limb defects (CHILD) [MIM:308050]. CHILD is an X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, which typically results in male lethality. Clinically, it is characterized by congenital, unilateral, ichthyosisform erythroderma with striking lateralization, sharp midline demarcation, and ipsilateral limb defects and hypoplasia of the body. Limbs defects range from hypoplasia of digits or ribs to complete amelia, often including scoliosis.
Defects in NSDHL are the cause of CK syndrome (CKS) [MIM:300831]. CKS is a disorder characterized by mild to severe cognitive impairment, seizures, microcephaly, cerebral cortical malformations, dysmorphic facial features, and thin body habitus. -
Sequence similarities
Belongs to the 3-beta-HSD family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 50814 Human
- Omim: 300275 Human
- SwissProt: Q15738 Human
- Unigene: 57698 Human
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Alternative names
- decarboxylating antibody
- H105E3 antibody
- H105e3 protein antibody
see all
Images
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Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)All lanes : Anti-NSDHL antibody [EPR14490] (ab190353) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NSDHL knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab190353 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.
ab190353 Anti-NSDHL antibody [EPR14490] was shown to specifically react with NSDHL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266682 (knockout cell lysate ab258082) was used. Wild-type and NSDHL knockout samples were subjected to SDS-PAGE. ab190353 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)](https://www.abcam.com/ps/products/190/ab190353/Images/ab190353-226221-ab1903533.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling NSDHL with ab190353 at 1/100 dilution. Secondary antibody HRP Polymer for Rabbit IgG prediluted. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)](https://www.abcam.com/ps/products/190/ab190353/Images/ab190353-226220-ab1903532.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NSDHL with ab190353 at 1/100 dilution. Secondary antibody HRP Polymer for Rabbit IgG prediluted. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (ab190353)](https://www.abcam.com/ps/products/190/ab190353/Images/ab190353-226222-ab1903534.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (ab190353)Immunofluorescence analysis of U87-MG cells, labeling NSDHL (red) with ab190353 at 1/50 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
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![Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)](https://www.abcam.com/ps/products/190/ab190353/Images/ab190353-226219-ab190353.jpg)
Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)All lanes : Anti-NSDHL antibody [EPR14490] (ab190353) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
![Anti-NSDHL antibody [EPR14490] (ab190353)](https://www.abcam.com/ps/products/190/ab190353/Images/ab190353-6-benefits-of-recombinant-antibodies.png)
Anti-NSDHL antibody [EPR14490] (ab190353)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab190353 has been referenced in 2 publications.
- Yoon SH et al. NAD(P)-dependent steroid dehydrogenase-like is involved in breast cancer cell growth and metastasis. BMC Cancer 20:375 (2020). PubMed: 32366230
- Ricco N et al. Mevalonate pathway activity as a determinant of radiation sensitivity in head and neck cancer. Mol Oncol 13:1927-1943 (2019). PubMed: 31225926
