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  1. Link

    nsdhl-antibody-epr14490-ab190353.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-NSDHL antibody [EPR14490] (ab190353)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)
  • Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (ab190353)
  • Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
  • Anti-NSDHL antibody [EPR14490] (ab190353)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14490] to NSDHL
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human NSDHL protein (ab162360)
Knockout
Product image
Human NSDHL knockout HEK-293T cell line (ab266682)

View more associated products

Overview

  • Product name

    Anti-NSDHL antibody [EPR14490]
    See all NSDHL primary antibodies
  • Description

    Rabbit monoclonal [EPR14490] to NSDHL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, A431, HeLa and Human fetal brain lysates. IHC-P: Human liver and Human adenocarcinoma of endometrium tissue. ICC: U87-MG cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14490
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism

Associated products

  • Alternative Versions

    • Anti-NSDHL antibody [EPR14490] - BSA and Azide free (ab251016)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human NSDHL knockout HEK-293T cell line (ab266682)
  • KO cell lysates

    • Human NSDHL knockout HEK-293T cell lysate (ab258082)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human NSDHL protein (ab162360)
  • Related Products

    • Recombinant Human NSDHL protein (ab162360)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 42 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 42 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50.

Target

  • Tissue specificity

    Brain, heart, liver, lung, kidney, skin and placenta.
  • Pathway

    Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.
  • Involvement in disease

    Defects in NSDHL are the cause of congenital hemidysplasia with ichthyosiform erythroderma and limb defects (CHILD) [MIM:308050]. CHILD is an X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, which typically results in male lethality. Clinically, it is characterized by congenital, unilateral, ichthyosisform erythroderma with striking lateralization, sharp midline demarcation, and ipsilateral limb defects and hypoplasia of the body. Limbs defects range from hypoplasia of digits or ribs to complete amelia, often including scoliosis.
    Defects in NSDHL are the cause of CK syndrome (CKS) [MIM:300831]. CKS is a disorder characterized by mild to severe cognitive impairment, seizures, microcephaly, cerebral cortical malformations, dysmorphic facial features, and thin body habitus.
  • Sequence similarities

    Belongs to the 3-beta-HSD family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q15738 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50814 Human
    • Omim: 300275 Human
    • SwissProt: Q15738 Human
    • Unigene: 57698 Human
    • Alternative names

      • decarboxylating antibody
      • H105E3 antibody
      • H105e3 protein antibody
      • NAD(P) dependent steroid dehydrogenase like antibody
      • NSDHL antibody
      • NSDHL_HUMAN antibody
      • Protein H105e3 antibody
      • SDR31E1 antibody
      • Short chain dehydrogenase/reductase family 31E member 1 antibody
      • Sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating antibody
      • Sterol-4-alpha-carboxylate 3-dehydrogenase antibody
      • XAP104 antibody
      see all

    Images

    • Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
      Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
      All lanes : Anti-NSDHL antibody [EPR14490] (ab190353) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : NSDHL knockout HEK293T cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 42 kDa
      Observed band size: 38 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab190353 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

       ab190353 Anti-NSDHL antibody [EPR14490]  was shown to specifically react with NSDHL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266682 (knockout cell lysate ab258082) was used. Wild-type and NSDHL knockout samples were subjected to SDS-PAGE. ab190353 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)

      Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling NSDHL with ab190353 at 1/100 dilution. Secondary antibody HRP Polymer for Rabbit IgG prediluted. Counter stain Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NSDHL with ab190353 at 1/100 dilution. Secondary antibody HRP Polymer for Rabbit IgG prediluted. Counter stain Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (ab190353)
      Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (ab190353)

      Immunofluorescence analysis of U87-MG cells, labeling NSDHL (red) with ab190353 at 1/50 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

    • Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
      Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
      All lanes : Anti-NSDHL antibody [EPR14490] (ab190353) at 1/10000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 42 kDa
      Observed band size: 38 kDa why is the actual band size different from the predicted?

    • Anti-NSDHL antibody [EPR14490] (ab190353)
      Anti-NSDHL antibody [EPR14490] (ab190353)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab190353? Please let us know so that we can cite the reference in this datasheet.

    ab190353 has been referenced in 2 publications.

    • Yoon SH  et al. NAD(P)-dependent steroid dehydrogenase-like is involved in breast cancer cell growth and metastasis. BMC Cancer 20:375 (2020). PubMed: 32366230
    • Ricco N  et al. Mevalonate pathway activity as a determinant of radiation sensitivity in head and neck cancer. Mol Oncol 13:1927-1943 (2019). PubMed: 31225926

    Customer reviews and Q&As

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