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  1. Link

    nse-antibody-ab139749.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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Anti-NSE antibody (ab139749)

  • Datasheet
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Western blot - Anti-NSE antibody (ab139749)
  • Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody (ab139749)

Key features and details

  • Chicken polyclonal to NSE
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgY

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-NSE antibody [EPR12483] (ab180943)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-NSE antibody
    See all NSE primary antibodies
  • Description

    Chicken polyclonal to NSE
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Goat, Chicken, Cow, Cat, Chimpanzee, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human NSE aa 400-500 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab140040)

  • Positive control

    • In Western Blot, ab139749 gave a positive signal in human fetal brain tissue lysate and in the following whole cell lysates: SHSY5Y ; HeLa; Y79. This antibody gave a positive result in Immunocytochemistry when used in the following methanol fixed cell lines: SKNSH.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 3% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Neuron Specific Enolase
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Neuroscience
    • Development

Associated products

  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant human NSE protein (ab168023)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities

    Belongs to the enolase family.
  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization

    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Target information above from: UniProt accession P09104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395689 Chicken
    • Entrez Gene: 526006 Cow
    • Entrez Gene: 2026 Human
    • Entrez Gene: 13807 Mouse
    • Entrez Gene: 24334 Rat
    • Omim: 131360 Human
    • SwissProt: O57391 Chicken
    • SwissProt: P09104 Human
    • SwissProt: P17183 Mouse
    • SwissProt: P07323 Rat
    • Unigene: 511915 Human
    • Unigene: 3913 Mouse
    • Unigene: 10828 Rat
    see all
  • Alternative names

    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Images

  • Western blot - Anti-NSE antibody (ab139749)
    Western blot - Anti-NSE antibody (ab139749)
    All lanes : Anti-NSE antibody (ab139749) at 1 µg/ml

    Lane 1 : Brain (Human) Cytoplasmic Lysate - fetal normal tissue
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139749 overnight at 4°C. Antibody binding was detected using an anti-chicken antibody conjugated to HRP, and visualised using ECL development solution.

  • Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody (ab139749)
    Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody (ab139749)

    ab139749 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab139749 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab139749? Please let us know so that we can cite the reference in this datasheet.

ab139749 has not yet been referenced specifically in any publications.

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