Anti-NSE antibody (ab15486)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-NSE antibody
See all NSE primary antibodies -
DescriptionRabbit polyclonal to NSE
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human NSE aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P09104 -
Positive control
- IMR cells. Pancreas.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab15486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 1/300 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 minutes, followed by cooling at RT for 20 minutes. |
Target
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FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
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Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
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Sequence similaritiesBelongs to the enolase family.
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Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
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Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
- Information by UniProt
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Database links
- Entrez Gene: 2026 Human
- Omim: 131360 Human
- SwissProt: P09104 Human
- Unigene: 511915 Human
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Alternative names
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody (ab15486)ab15486 staining NSE in human pancreas by Immunohistochemistry (FFPE-sections).
Protocols
Datasheets and documents
References
ab15486 has not yet been referenced specifically in any publications.
