Anti-NSE antibody (ab15486)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-NSE antibody
See all NSE primary antibodies -
DescriptionRabbit polyclonal to NSE
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-Pmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide within Human NSE aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P09104 -
Positive control
- IMR cells. Pancreas.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, BSA -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab15486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 1/300 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 minutes, followed by cooling at RT for 20 minutes. |
Target
-
FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
-
Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
-
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
-
Sequence similaritiesBelongs to the enolase family.
-
Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
-
Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
- Information by UniProt
-
Database links
- Entrez Gene: 2026 Human
- Omim: 131360 Human
- SwissProt: P09104 Human
- Unigene: 511915 Human
-
Alternative names
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody (ab15486)ab15486 staining NSE in human pancreas by Immunohistochemistry (FFPE-sections).
Datasheets and documents
Protocols
References
ab15486 has not yet been referenced specifically in any publications.
