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  1. Link

    nse-antibody-ab227301.pdf

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Anti-NSE antibody (ab227301)

  • Datasheet
  • SDS
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Western blot - Anti-NSE antibody (ab227301)
  • Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody (ab227301)
  • Immunohistochemistry (Frozen sections) - Anti-NSE antibody (ab227301)
  • Western blot - Anti-NSE antibody (ab227301)

Key features and details

  • Rabbit polyclonal to NSE
  • Suitable for: WB, IHC-Fr, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-NSE antibody
    See all NSE primary antibodies
  • Description

    Rabbit polyclonal to NSE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human NSE (internal sequence). The exact sequence is proprietary.
    Database link: P09104

  • Positive control

    • ICC/IF: A431 cells. IHC-Fr: E13.5 rat brain tissue. WB: Mouse brain lysate; HEK-293T and A431 whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Neuron Specific Enolase
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab30132)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (ab4022)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human NSE protein (ab168023)
  • Related Products

    • Recombinant human NSE protein (ab168023)
    • Recombinant Human NSE protein (ab78797)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
IHC-Fr
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
IHC-Fr
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities

    Belongs to the enolase family.
  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization

    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Target information above from: UniProt accession P09104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395689 Chicken
    • Entrez Gene: 526006 Cow
    • Entrez Gene: 2026 Human
    • Entrez Gene: 13807 Mouse
    • Entrez Gene: 24334 Rat
    • Entrez Gene: 714208 Rhesus monkey
    • Omim: 131360 Human
    • SwissProt: O57391 Chicken
    • SwissProt: P09104 Human
    • SwissProt: P17183 Mouse
    • SwissProt: P07323 Rat
    • Unigene: 511915 Human
    • Unigene: 3913 Mouse
    • Unigene: 10828 Rat
    see all
  • Alternative names

    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Images

  • Western blot - Anti-NSE antibody (ab227301)
    Western blot - Anti-NSE antibody (ab227301)
    Anti-NSE antibody (ab227301) at 1/10000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 47 kDa



    10% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody (ab227301)
    Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody (ab227301)

    A431 (human epidermoid carcinoma cell line) cells stained for NSE (green) using ab227301 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Frozen sections) - Anti-NSE antibody (ab227301)
    Immunohistochemistry (Frozen sections) - Anti-NSE antibody (ab227301)

    Frozen sectioned E13.5 rat brain tissue stained for NSE using ab227301 at 1/250 dilution in immunohistochemical analysis.

    Red: beta Tubulin 3, a mature neuron marker.
    Blue: DAPI.

  • Western blot - Anti-NSE antibody (ab227301)
    Western blot - Anti-NSE antibody (ab227301)
    All lanes : Anti-NSE antibody (ab227301) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS-PAGE gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab227301? Please let us know so that we can cite the reference in this datasheet.

ab227301 has not yet been referenced specifically in any publications.

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