Anti-NSE antibody (ab227301)
Key features and details
- Rabbit polyclonal to NSE
- Suitable for: WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NSE antibody
See all NSE primary antibodies -
Description
Rabbit polyclonal to NSE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human NSE (internal sequence). The exact sequence is proprietary.
Database link: P09104 -
Positive control
- ICC/IF: A431 cells. IHC-Fr: E13.5 rat brain tissue. WB: Mouse brain lysate; HEK-293T and A431 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 47 kDa. | |
| IHC-Fr | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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Database links
- Entrez Gene: 395689 Chicken
- Entrez Gene: 526006 Cow
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Entrez Gene: 24334 Rat
- Entrez Gene: 714208 Rhesus monkey
- Omim: 131360 Human
- SwissProt: O57391 Chicken
see all -
Alternative names
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
Images
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Western blot - Anti-NSE antibody (ab227301)Anti-NSE antibody (ab227301) at 1/10000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 47 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody (ab227301)A431 (human epidermoid carcinoma cell line) cells stained for NSE (green) using ab227301 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Frozen sections) - Anti-NSE antibody (ab227301)Frozen sectioned E13.5 rat brain tissue stained for NSE using ab227301 at 1/250 dilution in immunohistochemical analysis.
Red: beta Tubulin 3, a mature neuron marker.
Blue: DAPI. -
Western blot - Anti-NSE antibody (ab227301)All lanes : Anti-NSE antibody (ab227301) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227301 has not yet been referenced specifically in any publications.
