Anti-NSE antibody (ab227301)
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Overview
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Product name
Anti-NSE antibody
See all NSE primary antibodies -
Description
Rabbit polyclonal to NSE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human NSE (internal sequence). The exact sequence is proprietary.
Database link: P09104 -
Positive control
- ICC/IF: A431 cells. IHC-Fr: E13.5 rat brain tissue. WB: Mouse brain lysate; HEK-293T and A431 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 47 kDa. | |
| IHC-Fr | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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Database links
- Entrez Gene: 395689 Chicken
- Entrez Gene: 526006 Cow
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Entrez Gene: 24334 Rat
- Entrez Gene: 714208 Rhesus monkey
- Omim: 131360 Human
- SwissProt: O57391 Chicken
see all -
Alternative names
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
Images
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Western blot - Anti-NSE antibody (ab227301)Anti-NSE antibody (ab227301) at 1/10000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 47 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody (ab227301)A431 (human epidermoid carcinoma cell line) cells stained for NSE (green) using ab227301 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Frozen sections) - Anti-NSE antibody (ab227301)Frozen sectioned E13.5 rat brain tissue stained for NSE using ab227301 at 1/250 dilution in immunohistochemical analysis.
Red: beta Tubulin 3, a mature neuron marker.
Blue: DAPI. -
Western blot - Anti-NSE antibody (ab227301)All lanes : Anti-NSE antibody (ab227301) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa10% SDS-PAGE gel.
References
ab227301 has not yet been referenced specifically in any publications.
