Key features and details
- Rabbit polyclonal to NSE
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-NSE antibody
See all NSE primary antibodies
DescriptionRabbit polyclonal to NSE
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Recombinant fragment (His-tag) corresponding to Mouse NSE. Immunogen comprises aa1 - aa104 fused to aa224- aa434. N-terminal tag. Expressed in E. coli.
MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELR DGDKQRYLGKGVLKAVDHINSRIAPALISSGISVVEQEKLDNLMLELDGT ENKSLELVKEAIDKAGYTEKMVIGMDVAASEFYRDGKYDLDFKSPADPSR YITGDQLGALYQDFVRNYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDD LTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMV SHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGD EARFAGHNFRNPSVL
Database link: P17183
- WB: Recombinant mouse NSE; mouse muscle, heart and brain tissue lysates. IHC-P: Mouse brain tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography
Our Abpromise guarantee covers the use of ab231068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Sequence similaritiesBelongs to the enolase family.
Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
- Information by UniProt
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
Formalin-fixed, paraffin-embedded mouse brain tissue stained for NSE with ab231068 at 20 μg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-NSE antibody (ab231068) at 1 µg/ml
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse brain tissue lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Anti-NSE antibody (ab231068) at 2 µg/ml + Recombinant mouse NSE protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231068 has not yet been referenced specifically in any publications.