• Product name
  • Description
    Rabbit polyclonal to NSE
  • Host species
  • Specificity
    This antibody detects endogenous levels of total NSE protein.
  • Tested applications
    Suitable for: IHC-FoFr, Flow Cyt, ICC/IF, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide derived from human NSE

  • Positive control
    • Human brain tissue; extracts from HepG2 cells



Our Abpromise guarantee covers the use of ab53025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/75.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
IHC-P Use at an assay dependent concentration.


  • Function
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificity
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities
    Belongs to the enolase family.
  • Developmental stage
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization
    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all


  • Intracellular flow analysis of NSE on N2A mouse neuroblastoma cells using ab53025 at 1:500 dilution. Purple histogram represents rabbit IgG negative control; green line represents anti-NSE antibody (ab53025).

    See Abreview

  • ab53025 staining NSE in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3 Triton-X,  and blocked with 5% serum for 1 hour at room temperature. The sample was incubated with primary antibody (1/250) at 4°C for 16 hours. Ab96921, DyLight® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab53025 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53025, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53025 at 1/50-1/100 dilution. Left: without immunizing peptide; Right: with immunizing peptide
  • All lanes : Anti-NSE antibody (ab53025) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells with no immunizing peptide
    Lane 2 : Extracts from HepG2 cells with immunizing peptide

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • ab53025 staining NSE in mouse brain tissue section by Immunohistochemistry (PFA-perfussion fixed frozen tissue sections). Tissues were collected from mice in each group at 10 weeks. Mice were euthanized, the brains were exposed and removed from the body. Then the brain was cut, on ice and fixed in 4% polyformaldehyde or glutaraldehyde solution for histological examination. The primary antibody was used at 1/75 dilution in PBS and incubated with sample for 2 hours at 37°C. A HRP-conjugated secondary antibody was used at for 1hr at 37°C in a humidity box.


This product has been referenced in:
  • Li W  et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther 9:9 (2018). Read more (PubMed: 29335016) »
  • Ugras S  et al. Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in a-Synuclein Aggregation Disorders. EBioMedicine 31:307-319 (2018). WB ; Mouse . Read more (PubMed: 29759483) »

See all 24 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Dog Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1

Mr. Emmanuel Bouchaert

Verified customer

Submitted Mar 03 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: r.t°C
Antigen retrieval step
Rat Tissue sections (brain)
Yes - 0.3% Triton X

Mrs. Olga Cravetchi

Verified customer

Submitted Sep 11 2013

Thank you for contacting us.

It has been long time since you contacted us regarding the specificity of this antibody. Our laboratory has finally finished the testing of this antibody and can confirm the binding to RAW cells. Although the prot...

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Vielen Dank für Ihre Email.

Wenn Sie noch keinen RabMap bei uns bestellt haben, suchen Sie sich einfach einen aus :)

Meine Kollegen vom Kundendienst helfen Ihnen gerne bei der Bestellung.

Ich hoffe, dies hilft Ihnen...

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Ablaufdatum: 2013-03-27

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab53025 in Callithrix jacchus zu testen, annehmen möchten. Wie am Telefon besprochen können Sie den Gutscheincode zum Kauf...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Ich habe die Homologie der Immunogene mit Callithrix jacchus verglichen:

ab53025 : 100%
ab104225 : 96%

Unseres Wissens nach...

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Thank you very much for your patience.

I am still investigating this antibody with the developer laboratory. Unfortunately I still haven't got any concrete answer to your question.

I will get back to you soon with explanation.

Thank you for contacting us.

I apologies for the delay as I have missed your inquiry with others. I have sent an email to the developer laboratory and now waiting for a confirmation from them. I will send you the details soon.

Many t...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Brain and Pancreas)
Brain and Pancreas
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid HIER
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Oct 26 2011

1-10 of 15 Abreviews or Q&A


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