Overview

  • Product name
  • Description
    Rabbit polyclonal to NSE
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length human NSE purified from E. coli.

  • Positive control
    • WB: Rat brain and spinal cord lysate; Mouse brain and spinal cord lysate; NIH/3T3, HEK-293, HeLa, SH-SY5Y and C6 cell lysate. ICC/IF: Mixed cortical neuron-glial cell culture from E20 rat and HEP293 cells.
  • General notes
    The total protein content in serum: about 60mg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab64721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500.
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF 1/500. For optimal signal, fix with 3.7% formalin in PBS for one minute followed by 100% cold (-20C) methanol for one minute.

Target

  • Function
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificity
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities
    Belongs to the enolase family.
  • Developmental stage
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization
    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Images

  • All lanes : Anti-NSE antibody (ab64721) at 1/5000 dilution

    Lane 1 : Protein standard
    Lane 2 : Rat brain lysate
    Lane 3 : Rat spinal cord lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Mouse spinal cord lysate
    Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 7 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 8 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 9 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
    Lane 10 : C6 (Rat glial tumor cell line) cell lysate

    Predicted band size: 47 kDa

  • Immunofluorescence analysis of mixed cortical neuron-glial cell culture from E20 rat stained for NSE using ab64721 at a 1/500 dilution (red). Costained with chicken anti-GFAP at a 1/5000 dilution (green). The blue is Hoechst staining of nuclear DNA.

  • Immunocytochemistry/ Immunofluorescence of a mixed culture of cortical cells (4% PFA-fixation), with the red staining being GFAP, the green staining being NSE stained with ab64721, and the blue nuclear staining being DAPI.

  • Anti-NSE antibody (ab64721) + Adult male mouse brain at 10 mg/ml

    Predicted band size: 47 kDa

  • Immunofluorescent staining of human embryonic kidney cell line 293 by ab64721 (1:500). Hek293 expresses many neuronal proteins.

    The red channel shows staining with ab64721, which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker with a monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1), ab64722. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.

References

ab64721 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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