Anti-NSE antibody [ENO2/1375] (ab218388)

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Key features and details

  • Mouse monoclonal [ENO2/1375] to NSE
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-NSE antibody [ENO2/1375]
    See all NSE primary antibodies

  • Description

    Mouse monoclonal [ENO2/1375] to NSE

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken

  • Immunogen

    Synthetic peptide within Human NSE aa 416-433. The exact sequence is proprietary.
    Sequence:

    ELGDEARFAGHNFRNPSVL


    Database link: P09104

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human pheochromocytoma, cerebellum, mouse pancreas, and rat heart tissues. Y79, HepG2, and HeLa cell lysates.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab218388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 - 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(for 30 minutes at RT)
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 47 kDa.

Target

  • Function

    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

  • Sequence similarities

    Belongs to the enolase family.

  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

  • Cellular localization

    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pheochromocytoma tissue labeling NSE with ab218388 at 0.2 µg/mL.

  • Western blot - Anti-NSE antibody [ENO2/1375] (ab218388)
    Western blot - Anti-NSE antibody [ENO2/1375] (ab218388)
    All lanes : Anti-NSE antibody [ENO2/1375] (ab218388) at 1 µg/ml

    Lane 1 : Y79 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : HepG2 cell lysates

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebellum tissue labeling NSE with ab218388 at 0.2 µg/mL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse pancreas tissue labeling NSE with ab218388 at 0.2 µg/mL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling NSE with ab218388 at 0.2 µg/mL.

References (2)

Publishing research using ab218388? Please let us know so that we can cite the reference in this datasheet.

ab218388 has been referenced in 2 publications.

  • Thrivikraman G  et al. Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Nat Commun 10:3520 (2019). PubMed: 31388010
  • Feng X  et al. Spinal cord extracts from injured spinal cord impede differentiation of rat embryonic neural stem cells into neurons through regulating Notch signaling pathway. Int J Clin Exp Pathol 12:3855-3861 (2019). PubMed: 31933774

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