Anti-NSE antibody [ENO2/1375] (ab218388)

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Overview

  • Product name

    Anti-NSE antibody [ENO2/1375]
    See all NSE primary antibodies

  • Description

    Mouse monoclonal [ENO2/1375] to NSE

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken

  • Immunogen

    Synthetic peptide within Human NSE aa 416-433. The exact sequence is proprietary.
    Sequence:

    ELGDEARFAGHNFRNPSVL


    Database link: P09104

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human pheochromocytoma, cerebellum, mouse pancreas, and rat heart tissues. Y79, HepG2, and HeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab218388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 - 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(for 30 minutes at RT)
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 47 kDa.

Target

  • Function

    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

  • Sequence similarities

    Belongs to the enolase family.

  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

  • Cellular localization

    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pheochromocytoma tissue labeling NSE with ab218388 at 0.2 µg/mL.

  • Western blot - Anti-NSE antibody [ENO2/1375] (ab218388)
    Western blot - Anti-NSE antibody [ENO2/1375] (ab218388)
    All lanes : Anti-NSE antibody [ENO2/1375] (ab218388) at 1 µg/ml

    Lane 1 : Y79 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : HepG2 cell lysates

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebellum tissue labeling NSE with ab218388 at 0.2 µg/mL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse pancreas tissue labeling NSE with ab218388 at 0.2 µg/mL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling NSE with ab218388 at 0.2 µg/mL.

References

This product has been referenced in:

  • Thrivikraman G  et al. Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Nat Commun 10:3520 (2019). Read more (PubMed: 31388010) »
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