Anti-NSE antibody [ENO2/1375] (ab218388)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)](https://www.abcam.com/ps/products/218/ab218388/Images/ab218388-273670-anti-nse-antibody-eno2-1375-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)")
Key features and details
- Mouse monoclonal [ENO2/1375] to NSE
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-NSE antibody [ENO2/1375]
See all NSE primary antibodies -
Description
Mouse monoclonal [ENO2/1375] to NSE -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NSE aa 400 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P09104 -
Positive control
- Human pheochromocytoma, cerebellum, mouse pancreas, and rat heart tissues. Y79, HepG2, and HeLa cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab218388 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
ENO2/1375 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.2 - 0.4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 47 kDa.
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| Notes |
|---|
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IHC-P
Use a concentration of 0.2 - 0.4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 47 kDa. |
Target
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Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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Database links
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Entrez Gene: 24334 Rat
- Omim: 131360 Human
- SwissProt: P09104 Human
- SwissProt: P17183 Mouse
- SwissProt: P07323 Rat
- Unigene: 511915 Human
see all -
Alternative names
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pheochromocytoma tissue labeling NSE with ab218388 at 0.2 µg/mL.
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![Western blot - Anti-NSE antibody [ENO2/1375] (ab218388)](https://www.abcam.com/ps/products/218/ab218388/Images/ab218388-273666-anti-nse-antibody-eno2-1375-western-blot.jpg)
Western blot - Anti-NSE antibody [ENO2/1375] (ab218388)All lanes : Anti-NSE antibody [ENO2/1375] (ab218388) at 1 µg/ml
Lane 1 : Y79 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : HepG2 cell lysates
Predicted band size: 47 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)](https://www.abcam.com/ps/products/218/ab218388/Images/ab218388-273669-anti-nse-antibody-eno2-1375-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebellum tissue labeling NSE with ab218388 at 0.2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)](https://www.abcam.com/ps/products/218/ab218388/Images/ab218388-273668-anti-nse-antibody-eno2-1375-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse pancreas tissue labeling NSE with ab218388 at 0.2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)](https://www.abcam.com/ps/products/218/ab218388/Images/ab218388-273667-anti-nse-antibody-eno2-1375-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling NSE with ab218388 at 0.2 µg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab218388 has been referenced in 3 publications.
- Liu H et al. Olig2 SUMOylation protects against genotoxic damage response by antagonizing p53 gene targeting. Cell Death Differ N/A:N/A (2020). PubMed: 32483381
- Thrivikraman G et al. Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Nat Commun 10:3520 (2019). PubMed: 31388010
- Feng X et al. Spinal cord extracts from injured spinal cord impede differentiation of rat embryonic neural stem cells into neurons through regulating Notch signaling pathway. Int J Clin Exp Pathol 12:3855-3861 (2019). PubMed: 31933774
