• Product name
    Anti-NSE antibody [EPR3377]
    See all NSE primary antibodies
  • Description
    Rabbit monoclonal [EPR3377] to NSE
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NSE aa 1-100. The exact sequence is proprietary.

  • Positive control
    • WB: Human fetal brain tissue lysate and SH-SY-5Y, HeLa and Y79 cell lysates. IHC-P: Human brain tissue. ICC/IF: formaldehyde fixed SKNSH cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab79757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/5000 - 1/20000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Flow Cyt 1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.


  • Function
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificity
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities
    Belongs to the enolase family.
  • Developmental stage
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization
    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all


  • All lanes : Anti-NSE antibody [EPR3377] (ab79757) at 1/20000 dilution

    Lane 1 : fetal brain lysate
    Lane 2 : SH-SY-5Y lysate
    Lane 3 : HeLa lysate
    Lane 4 : Y79 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/250-1/500 ab79757.
  • ICC/IF image of ab79757 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79757 at 1/25 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing HeLa cells stained with ab79757 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79757, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.


This product has been referenced in:
  • Yu P  et al. Neurotropism In Vitro and Mouse Models of Severe and Mild Infection with Clinical Strains of Enterovirus 71. Viruses 9:N/A (2017). Read more (PubMed: 29156632) »
  • Park T  et al. Overexpression of Neuron-Specific Enolase as a Prognostic Factor in Patients with Gastric Cancer. J Gastric Cancer 17:228-236 (2017). Read more (PubMed: 28970953) »
See all 8 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
Cryopreserved embryonic cortical neurons (QBMcells
paraformaldehyde with picric acid

Ms. Babben Tinner

Verified customer

Submitted Nov 06 2015

Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Rat Tissue lysate - other (hippocampus)
Blocking step
Milk as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 25°C

Mr. House Liu

Verified customer

Submitted Nov 07 2014

Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Zebrafish Tissue sections (retina, neurons)
retina, neurons
Yes - triton X

Abcam user community

Verified customer

Submitted Sep 05 2014


I'm sorry about the miss-alignment and thank you for spotting it.

We have a testing discount scheme which you would be eligible to try. This would involve you purchasing ab79757, testing it with your Drosophila samples, and sharing the results with us in the form of an Abreview. Your would then be eligible for a free primary antibody of your choice from our catalogue. More information on this scheme can be found from the link below:


If you would be interested in participating in this scheme please do let me know and I will issue you with a discount code.

Read More


Thank you for contacting us.

The immunogen used to raise anti-NSE antibody (ab79757) is taken from within the region of of human 35 - 60 of enolase2 gamma (NSE) (UniProt: P09104). From my analysis, this shares some homology within the N-terminal region but matches poorly within the C-terminal region with the Drosophila enolase sequence I identified (SwissProt reference P15007), please see the attachment of this email. However, I am not sure this is the sequence you are interested in, do you have an alternative sequence which I can perform the analysis?

If this Drosophila sequence is correct, I would suggest that it may be unlikely that antibody is able to recognise the sequence.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More


Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Human breast cancer 2.Anti-Progesterone Receptor antibody [SP2] (ab16661) Human breast carcinoma 3.Anti-Estrogen Receptor antibody [SP1] (ab16660) Human breast carcinoma 4.Anti-Myeloperoxidase antibody (ab45977) Human spleen tissue 5.Anti-TdT antibody (ab85148) Human testis 6.Anti-TMEM16A antibody (ab53212) Human liver 7.Anti-NSE antibody [EPR3377] (ab79757) Human brain tissue 8.Anti-CD23 antibody [EPR3617(IHC)] (ab92495) Human tonsil 9.Anti-CD10 antibody [56C6] (ab951) Lymph node tissue or human breast microepithelium 10.Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) Murine melanoma metastasis tissue 11.Anti-CD79a antibody [HM47/A9] (ab3121) Human Lymphoid tissue, tonsil 12.Anti-CD45RO antibody [UCHL-1] (ab86080) Human Tonsil, Thymus 13.Anti-CD45 antibody (ab10559) Human Tonsil tissue 14.Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) Human Hodgekins lymphoma tissue 15.Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Human Hodgekins lymphoma tissue Human spleen I am providing a list ofslides that we sell which I hope will be helpful to you. I can recommend to review the online datasheet for more details. Human brain: ab4305Brain (human): hippocampus normal tissue slides https://www.abcam.com/index.html?datasheet=4305 (or use the following: https://www.abcam.com/index.html?datasheet=4305). Human lymph node: ab4350Lymph node (human) normal tissue slides IHC-P, ISH https://www.abcam.com/index.html?datasheet=4350 (or use the following: https://www.abcam.com/index.html?datasheet=4350). Human testis: ab4373Testis (human) normal tissue slides IHC-P, ISH https://www.abcam.com/index.html?datasheet=4373 (or use the following: https://www.abcam.com/index.html?datasheet=4373). Human liver: ab4348Liver (human) normal tissue slides IHC-P, ISH https://www.abcam.com/index.html?datasheet=4348 (or use the following: https://www.abcam.com/index.html?datasheet=4348). Human spleen: ab4372Spleen (human) normal tissue slides IHC-P, ISH https://www.abcam.com/index.html?datasheet=4372 (or use the following: https://www.abcam.com/index.html?datasheet=4372). I am sorry we do not have slides forthe following: Human tonsil Hodgekins lymphoma tissue Mouse melenoma tissue I hope this will be helpful to you. If you have any further questions,please do not hesitate to contact us.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up