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RecombinantRabMAb

Recombinant Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

Submit a review Submit a question References (5)
Western blot - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Immunocytochemistry - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Flow Cytometry (Intracellular) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3377] to NSE - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 PE Unconjugated

Overview

  • Product name

    Anti-NSE antibody [EPR3377] - BSA and Azide free
    See all NSE primary antibodies

  • Description

    Rabbit monoclonal [EPR3377] to NSE - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: U-87 MG cells; IHC-P: Human, mouse and rat cerebrum tissue; Flow Cyt (intra): HeLa cells. WB: SH-SY5Y, HeLa, Y76 whole cell lysate, Mouse and rat brain lysate.

  • General notes

    ab220216 is the carrier-free version of ab79757.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab220216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • Function

    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

  • Sequence similarities

    Belongs to the enolase family.

  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

  • Cellular localization

    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Images

  • Western blot - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Western blot - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    All lanes : Anti-NSE antibody [EPR3377] (ab79757) at 1/1000 dilution (Purified)

    Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Y79 (Human retinoblastoma retinoblastoma) whole cell lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Rat brain lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    This data was developed using ab79757, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling NSE with purified ab79757 at 1/500 dilution (0.20 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunocytochemistry - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Immunocytochemistry - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    This data was developed using ab79757, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling NSE with purified ab79757 at 1/50 dilution (2.0 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Flow Cytometry (Intracellular) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    This data was developed using ab79757, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling NSE with purified ab79757 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    This data was developed using ab79757, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling NSE with purified ab79757 at 1/500 dilution (0.20 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    This data was developed using ab79757, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling NSE with purified ab79757 at 1/500 dilution (0.20 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

References (5)

Publishing research using ab220216? Please let us know so that we can cite the reference in this datasheet.

ab220216 has been referenced in 5 publications.

  • Zhang J  et al. AP4M1 is abnormally expressed in oxygen-glucose deprived hippocampal neurons. Neurosci Lett 563:85-9 (2014). IF ; Rat . PubMed: 24486887
  • Fishbein I  et al. Augmentation of phenotype in a transgenic Parkinson mouse heterozygous for a Gaucher mutation. Brain 137:3235-47 (2014). PubMed: 25351739
  • Meng X  et al. Cloning and expression of neuron-specific enolase in the corpus luteum of dairy goats. Gene 503:222-8 (2012). IF ; Goat . PubMed: 22575614
  • Soh MA  et al. Arsenic, cadmium and neuron specific enolase (ENO2, ?-enolase) expression in breast cancer. Cancer Cell Int 11:41 (2011). IHC-P ; Human . PubMed: 22098917
  • Zhang J  et al. Labeling primary nerve stem cells with quantum dots. J Nanosci Nanotechnol 11:9536-42 (2011). PubMed: 22413243

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