RecombinantRabMAb

Recombinant Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

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Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Flow Cytometry - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
  • Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3377] to NSE - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NSE antibody [EPR3377] - BSA and Azide free
    See all NSE primary antibodies

  • Description

    Rabbit monoclonal [EPR3377] to NSE - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    A synthetic peptide corresponding to residues in human NSE

  • Positive control

    • WB: Human fetal brain tissue lysate and SH-SY-5Y, HeLa and Y79 cell lysates. IHC-P: Human brain tissue. ICC/IF: formaldehyde fixed SKNSH cells.

  • General notes

    ab220216 is the carrier-free version of ab79757. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab220216 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab220216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • Function

    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

  • Sequence similarities

    Belongs to the enolase family.

  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

  • Cellular localization

    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    ICC/IF image of ab79757 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79757 at 1/25 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79757).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/250-1/500 ab79757.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79757).

  • Flow Cytometry - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Flow Cytometry - Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

    Overlay histogram showing HeLa cells stained with ab79757 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79757, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab79757).

  • Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)

References (5)

Publishing research using ab220216? Please let us know so that we can cite the reference in this datasheet.

ab220216 has been referenced in 5 publications.

  • Zhang J  et al. AP4M1 is abnormally expressed in oxygen-glucose deprived hippocampal neurons. Neurosci Lett 563:85-9 (2014). IF ; Rat . PubMed: 24486887
  • Fishbein I  et al. Augmentation of phenotype in a transgenic Parkinson mouse heterozygous for a Gaucher mutation. Brain 137:3235-47 (2014). PubMed: 25351739
  • Meng X  et al. Cloning and expression of neuron-specific enolase in the corpus luteum of dairy goats. Gene 503:222-8 (2012). IF ; Goat . PubMed: 22575614
  • Soh MA  et al. Arsenic, cadmium and neuron specific enolase (ENO2, ?-enolase) expression in breast cancer. Cancer Cell Int 11:41 (2011). IHC-P ; Human . PubMed: 22098917
  • Zhang J  et al. Labeling primary nerve stem cells with quantum dots. J Nanosci Nanotechnol 11:9536-42 (2011). PubMed: 22413243

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