Overview

  • Product name

    Anti-NSE antibody [EPR3378]
    See all NSE primary antibodies
  • Description

    Rabbit monoclonal [EPR3378] to NSE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NSE aa 350-450. The exact sequence is proprietary.

  • Positive control

    • Fetal brain, SH-SY5Y, Y79, U87-MG, HeLa and HepG2 cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
    • Tissue specificity

      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
    • Sequence similarities

      Belongs to the enolase family.
    • Developmental stage

      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
    • Cellular localization

      Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2 phospho D glycerate hydrolyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Eno 2 antibody
      • ENO2 antibody
      • ENOG antibody
      • ENOG_HUMAN antibody
      • Enolase 2 (gamma, neuronal) antibody
      • Enolase 2 antibody
      • Enolase 2 gamma neuronal antibody
      • Enolase2 antibody
      • Epididymis secretory protein Li 279 antibody
      • Gamma enolase antibody
      • Gamma-enolase antibody
      • HEL S 279 antibody
      • Neural enolase antibody
      • Neuron specific enolase antibody
      • Neuron specific gamma enolase antibody
      • Neuron-specific enolase antibody
      • neuronal enriched enolase antibody
      • Neurone specific enolase antibody
      • NSE antibody
      see all

    Images

    • All lanes : Anti-NSE antibody [EPR3378] (ab133309) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Y79 cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : HeLa cell lysate
      Lane 6 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    References

    ab133309 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133309.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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