RecombinantRabMAb

Recombinant Anti-NSE antibody [EPR3378] (ab133309)

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Western blot - Anti-NSE antibody [EPR3378] (ab133309)
  • Anti-NSE antibody [EPR3378] (ab133309)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3378] to NSE
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-NSE antibody [EPR3378]
    See all NSE primary antibodies

  • Description

    Rabbit monoclonal [EPR3378] to NSE

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human NSE aa 350-450. The exact sequence is proprietary.

  • Positive control

    • Fetal brain, SH-SY5Y, Y79, U87-MG, HeLa and HepG2 cell lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
Application notes
Is unsuitable for ICC,IHC-P or IP.

Target

  • Function

    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

  • Sequence similarities

    Belongs to the enolase family.

  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

  • Cellular localization

    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Images

  • Western blot - Anti-NSE antibody [EPR3378] (ab133309)
    Western blot - Anti-NSE antibody [EPR3378] (ab133309)
    All lanes : Anti-NSE antibody [EPR3378] (ab133309) at 1/1000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Y79 cell lysate
    Lane 4 : U87-MG cell lysate
    Lane 5 : HeLa cell lysate
    Lane 6 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 47 kDa

  • Anti-NSE antibody [EPR3378] (ab133309)
    Anti-NSE antibody [EPR3378] (ab133309)

References (0)

Publishing research using ab133309? Please let us know so that we can cite the reference in this datasheet.

ab133309 has not yet been referenced specifically in any publications.

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