Recombinant Anti-NSE antibody [EPR3378] (ab133309)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3378] to NSE
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NSE antibody [EPR3378]
See all NSE primary antibodies -
Description
Rabbit monoclonal [EPR3378] to NSE -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NSE aa 350-450. The exact sequence is proprietary.
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Positive control
- Fetal brain, SH-SY5Y, Y79, U87-MG, HeLa and HepG2 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3378 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 47 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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Database links
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Entrez Gene: 24334 Rat
- Omim: 131360 Human
- SwissProt: P09104 Human
- SwissProt: P17183 Mouse
- SwissProt: P07323 Rat
- Unigene: 511915 Human
see all -
Alternative names
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
Images
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![Western blot - Anti-NSE antibody [EPR3378] (ab133309)](https://www.abcam.com/ps/products/133/ab133309/Images/ab133309-146800-anti-nse-antibody-epr3378-western-blot.jpg)
Western blot - Anti-NSE antibody [EPR3378] (ab133309)All lanes : Anti-NSE antibody [EPR3378] (ab133309) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Y79 cell lysate
Lane 4 : U87-MG cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa
References
ab133309 has not yet been referenced specifically in any publications.
