Anti-NSE antibody - Neuronal Marker (ab53025)
Key features and details
- Rabbit polyclonal to NSE - Neuronal Marker
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NSE antibody - Neuronal Marker
See all NSE primary antibodies -
Description
Rabbit polyclonal to NSE - Neuronal Marker -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total NSE protein. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide derived from human NSE
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab53025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 47 kDa. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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Database links
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Omim: 131360 Human
- SwissProt: P09104 Human
- SwissProt: P17183 Mouse
- Unigene: 511915 Human
- Unigene: 3913 Mouse
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Alternative names
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
Images
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Western blot - Anti-NSE antibody - Neuronal Marker (ab53025)All lanes : Anti-NSE antibody - Neuronal Marker (ab53025) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells with no immunizing peptide
Lane 2 : Extracts from HepG2 cells with immunizing peptide
Predicted band size: 47 kDa
Observed band size: 47 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody - Neuronal Marker (ab53025)ICC/IF image of ab53025 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53025, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab53025)Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53025 at 1/50-1/100 dilution. Left: without immunizing peptide; Right: with immunizing peptide -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab53025)Image from Yu Yang. et. al. Mol. Cell. Proteomics, Mar 2009 (Fig 5).ab53025 staining NSE in mouse brain tissue section by Immunohistochemistry (PFA-perfussion fixed frozen tissue sections). Tissues were collected from mice in each group at 10 weeks. Mice were euthanized, the brains were exposed and removed from the body. Then the brain was cut, on ice and fixed in 4% polyformaldehyde or glutaraldehyde solution for histological examination. The primary antibody was used at 1/75 dilution in PBS and incubated with sample for 2 hours at 37°C. A HRP-conjugated secondary antibody was used at for 1hr at 37°C in a humidity box.
Protocols
Datasheets and documents
References (46)
ab53025 has been referenced in 46 publications.
- Evans HT et al. Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation. Elife 9:N/A (2020). PubMed: 31904341
- Chen X et al. CX3CL1/CX3CR1 axis attenuates early brain injury via promoting the delivery of exosomal microRNA-124 from neuron to microglia after subarachnoid hemorrhage. J Neuroinflammation 17:209 (2020). PubMed: 32664984
- Xu J et al. Self-Assembling Peptide Scaffold Carrying Neural-Cell Adhesion Molecule-Derived Mimetic-Peptide Transplantation Promotes Proliferation and Stimulates Neurite Extension by Modulating Tau Phosphorylation and Calpain/Glycogen Synthase Kinase 3 beta (GSK-3ß) in Neurons. Ann Transplant 25:e924093 (2020). PubMed: 32686658
- Karakas N et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
- Tiwari R et al. Androgen deprivation upregulates SPINK1 expression and potentiates cellular plasticity in prostate cancer. Nat Commun 11:384 (2020). PubMed: 31959826
