Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain and Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid HIER
Permeabilization
No
Specification
Brain and Pancreas
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
It seems to me that the brain positivity is appropriate (upper image).
However, the Islet of Langerhans positivity is, in my hands, limited to a sub-population of Islet cells (lower image) that does not conform to published data (which seem to show that most or all of the Islet cells are positive. However, there may be species/temporal expression differentials).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
Submitted Oct 26 2011