Anti-NSE antibody - Neuronal Marker (ab834)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)

    Key features and details

    • Rabbit polyclonal to NSE - Neuronal Marker
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-NSE antibody - Neuronal Marker
      See all NSE primary antibodies

    • Description

      Rabbit polyclonal to NSE - Neuronal Marker

    • Host species

      Rabbit

    • Specificity

      This antibody reacts with human isoenzymes of neuron specific enolase containing gamma subunits. This antibody gives a specific staining in neurons of the brain, in islets of langerhans in pancreas and in peripheral nerves of the skin. No staining with other tissue types has been seen.

    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data

    • Immunogen

      Neuron specific enolase (gamma-gamma isoenzyme) isolated from bovine brain.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab834 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    Notes
  • Application notes
    IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.

    • Target

    • Function

      Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

    • Tissue specificity

      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

    • Sequence similarities

      Belongs to the enolase family.

    • Developmental stage

      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

    • Cellular localization

      Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
    • Information by UniProt

    • Database links

      • Alternative names

        • 2 phospho D glycerate hydrolyase antibody
        • 2-phospho-D-glycerate hydro-lyase antibody
        • Eno 2 antibody
        • ENO2 antibody
        • ENOG antibody
        • ENOG_HUMAN antibody
        • Enolase 2 (gamma, neuronal) antibody
        • Enolase 2 antibody
        • Enolase 2 gamma neuronal antibody
        • Enolase2 antibody
        • Epididymis secretory protein Li 279 antibody
        • Gamma enolase antibody
        • Gamma-enolase antibody
        • HEL S 279 antibody
        • Neural enolase antibody
        • Neuron specific enolase antibody
        • Neuron specific gamma enolase antibody
        • Neuron-specific enolase antibody
        • neuronal enriched enolase antibody
        • Neurone specific enolase antibody
        • NSE antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)
        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, staining NSE - Neuronal Marker with ab834.

      References (1)

      Publishing research using ab834? Please let us know so that we can cite the reference in this datasheet.

      ab834 has been referenced in 1 publication.

      • Tang YY  et al. Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells via the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury. Biol Pharm Bull 40:1630-1637 (2017). PubMed: 28966235

      Customer reviews and Q&As

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      Question

      Is NSE sensitive to triton-X? Should I be leaving this out of my IHC protocol, or can I leave it in?

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      Answer

      We recommend the use of 0.025% Triton in the TBS as it helps to reduce surface tension, allowing reagents to cover the whole tissue section with ease. It is also believed to dissolve Fc receptors, therefore reducing non-specific binding. Furthermore, TBS gives a cleaner background than PBS. Care must be taken using Triton when working with membrane-bound proteins as the Triton acts as a surfactant and strips the lipid component out of the membrane. Neuron specific enolase (NSE) is located in the cytoplasm of mature neurons so you should have no problem using Triton in your buffer.

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