Anti-NSE antibody - Neuronal Marker (ab834)

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Overview

  • Product name
    Anti-NSE antibody - Neuronal Marker
    See all NSE primary antibodies
  • Description
    Rabbit polyclonal to NSE - Neuronal Marker
  • Host species
    Rabbit
  • Specificity
    This antibody reacts with human isoenzymes of neuron specific enolase containing gamma subunits. This antibody gives a specific staining in neurons of the brain, in islets of langerhans in pancreas and in peripheral nerves of the skin. No staining with other tissue types has been seen.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Neuron specific enolase (gamma-gamma isoenzyme) isolated from bovine brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notes
    IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.

    • Target

    • Function
      Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
    • Tissue specificity
      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
    • Pathway
      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
    • Sequence similarities
      Belongs to the enolase family.
    • Developmental stage
      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
    • Cellular localization
      Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
    • Information by UniProt
    • Database links
      • Alternative names
        • 2 phospho D glycerate hydrolyase antibody
        • 2-phospho-D-glycerate hydro-lyase antibody
        • Eno 2 antibody
        • ENO2 antibody
        • ENOG antibody
        • ENOG_HUMAN antibody
        • Enolase 2 (gamma, neuronal) antibody
        • Enolase 2 antibody
        • Enolase 2 gamma neuronal antibody
        • Enolase2 antibody
        • Epididymis secretory protein Li 279 antibody
        • Gamma enolase antibody
        • Gamma-enolase antibody
        • HEL S 279 antibody
        • Neural enolase antibody
        • Neuron specific enolase antibody
        • Neuron specific gamma enolase antibody
        • Neuron-specific enolase antibody
        • neuronal enriched enolase antibody
        • Neurone specific enolase antibody
        • NSE antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)
        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, staining NSE - Neuronal Marker with ab834.

      References

      ab834 has not yet been referenced specifically in any publications.

      Publishing research using ab834? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1 Review or Q&A

      10
      Votes: Highest First

      Is NSE sensitive to triton-X? Should I be leaving this out of my IHC protocol, or can I leave it in?

      We recommend the use of 0.025% Triton in the TBS as it helps to reduce surface tension, allowing reagents to cover the whole tissue section with ease. It is also believed to dissolve Fc receptors, therefore reducing non-specific binding. Furthermore, T...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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