Anti-NSE antibody - Neuronal Marker (ab834)
Key features and details
- Rabbit polyclonal to NSE - Neuronal Marker
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-NSE antibody - Neuronal Marker
See all NSE primary antibodies -
Description
Rabbit polyclonal to NSE - Neuronal Marker -
Host species
Rabbit -
Specificity
This antibody reacts with human isoenzymes of neuron specific enolase containing gamma subunits. This antibody gives a specific staining in neurons of the brain, in islets of langerhans in pancreas and in peripheral nerves of the skin. No staining with other tissue types has been seen. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Bovine NSE. Neuron specific enolase (gamma-gamma isoenzyme) isolated from bovine brain.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
| Notes |
|---|
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Target
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Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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Database links
- Entrez Gene: 2026 Human
- Omim: 131360 Human
- SwissProt: P09104 Human
- Unigene: 511915 Human
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Alternative names
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, staining NSE - Neuronal Marker with ab834.
Datasheets and documents
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Datasheet download
References (1)
ab834 has been referenced in 1 publication.
- Tang YY et al. Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells via the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury. Biol Pharm Bull 40:1630-1637 (2017). PubMed: 28966235
