Anti-NSFL1C antibody (ab95963)
Key features and details
- Rabbit polyclonal to NSFL1C
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NSFL1C antibody -
Description
Rabbit polyclonal to NSFL1C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant protein fragment with a sequence corresponding to a region within amino acids 21 and 272 of Human NSFL1C (NP_057227).
-
Positive control
- A431 whole cell lysate (ab7909) can be used as a positive control in WB.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
|
IHC-P |
1/100 - 1/250.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
IHC-P
1/100 - 1/250. |
Target
-
Function
Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). -
Sequence similarities
Belongs to the NSFL1C family.
Contains 1 SEP domain.
Contains 1 UBX domain. -
Post-translational
modificationsPhosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis. -
Cellular localization
Nucleus. Golgi apparatus > Golgi stack. Chromosome. Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks. - Information by UniProt
-
Database links
- Entrez Gene: 526612 Cow
- Entrez Gene: 55968 Human
- Entrez Gene: 386649 Mouse
- Entrez Gene: 83809 Rat
- Omim: 606610 Human
- SwissProt: Q9UNZ2 Human
- SwissProt: Q9CZ44 Mouse
- SwissProt: O35987 Rat
see all -
Alternative names
- DJ776F14.1 antibody
- MGC3347 antibody
- NSF1C_HUMAN antibody
see all
Images
-
All lanes : Anti-NSFL1C antibody (ab95963) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa -
ab95963, at 1/100, staining paraffin-embedded differential N/T pair Lung SCC cancer by Immunohistochemistry.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab95963 has been referenced in 3 publications.
- Her NG et al. p97 Composition Changes Caused by Allosteric Inhibition Are Suppressed by an On-Target Mechanism that Increases the Enzyme's ATPase Activity. Cell Chem Biol 23:517-28 (2016). PubMed: 27105284
- Davis EJ et al. DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. Nat Struct Mol Biol 19:1093-100 (2012). WB ; Human . PubMed: 23042607
- Mosbech A et al. DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. Nat Struct Mol Biol 19:1084-92 (2012). WB ; Human . PubMed: 23042605