Product nameAnti-NSFL1C antibody
See all NSFL1C primary antibodies
DescriptionRabbit polyclonal to NSFL1C
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant protein fragment with a sequence corresponding to a region within amino acids 21 and 272 of Human NSFL1C (NP_057227).
- A431 whole cell lysate (ab7909) can be used as a positive control in WB.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab95963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 41 kDa.|
|IHC-P||1/100 - 1/250.|
FunctionReduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro).
Sequence similaritiesBelongs to the NSFL1C family.
Contains 1 SEP domain.
Contains 1 UBX domain.
modificationsPhosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis.
Cellular localizationNucleus. Golgi apparatus > Golgi stack. Chromosome. Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks.
- Information by UniProt
- DJ776F14.1 antibody
- MGC3347 antibody
- NSF1C_HUMAN antibody
All lanes : Anti-NSFL1C antibody (ab95963) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
ab95963, at 1/100, staining paraffin-embedded differential N/T pair Lung SCC cancer by Immunohistochemistry.
ab95963 has been referenced in 3 publications.
- Her NG et al. p97 Composition Changes Caused by Allosteric Inhibition Are Suppressed by an On-Target Mechanism that Increases the Enzyme's ATPase Activity. Cell Chem Biol 23:517-28 (2016). PubMed: 27105284
- Davis EJ et al. DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. Nat Struct Mol Biol 19:1093-100 (2012). WB ; Human . PubMed: 23042607
- Mosbech A et al. DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. Nat Struct Mol Biol 19:1084-92 (2012). WB ; Human . PubMed: 23042605