Recombinant Anti-NSG2 antibody [EPR15389(B)] - BSA and Azide free (ab250989)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15389(B)] to NSG2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NSG2 antibody [EPR15389(B)] - BSA and Azide free
See all NSG2 primary antibodies -
Description
Rabbit monoclonal [EPR15389(B)] to NSG2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NSG2 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Y328 -
General notes
Ab250989 is the carrier-free version of ab189513. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250989 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR15389(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab250989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
Target
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Sequence similarities
Belongs to the NSG family. -
Cellular localization
Cytoplasmic vesicle membrane. Golgi apparatus > Golgi stack membrane. Endosome > multivesicular body membrane. Mainly Golgi stack, but also found in small vacuolar organelles and multivesicular bodies. - Information by UniProt
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Database links
- Entrez Gene: 51617 Human
- Entrez Gene: 18197 Mouse
- Entrez Gene: 497878 Rat
- SwissProt: Q9Y328 Human
- SwissProt: P47759 Mouse
- Unigene: 559412 Human
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Alternative names
- HMP19 antibody
- Neuron-specific protein family member 2 antibody
- Nsg2 antibody
see all
Images
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All lanes : Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513) at 1/1000 dilution
Lane 1 : Neuro-2a cell lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 19 kDaThis data was developed using ab189513, the same antibody clone in a different buffer formulation.
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All lanes : Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 19 kDaThis data was developed using ab189513, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab250989 has not yet been referenced specifically in any publications.