Key features and details
- Rabbit polyclonal to NSL1
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NSL1 antibody
See all NSL1 primary antibodies
DescriptionRabbit polyclonal to NSL1
Tested applicationsSuitable for: IHC-P, IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between amino acids 150-200 of Human NSL1 (NP_056286.2)
- Whole cell lysate from HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPart of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
- Information by UniProt
- 4833432M17Rik antibody
- AI451597 antibody
- C1orf48 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NSL1 with ab70835 at 1/1000 (1µg/ml). Detection: DAB.
Detection of Human NSL1 by Western Blot of Immunoprecipitates in Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
Lane 1: ab70835 used for IP at 3 µg/mg lysate.
Lane 2: immunoprecipitation with an other antibody
Lane 3: ab70836 used for IP at 3 µg/mg lysate
Lane 4: Control IgG IP
Detection: Chemiluminescence with an exposure time of 3 minutes. For blotting ab70836 was used at 1 µg/ml.
ab70835 has not yet been referenced specifically in any publications.