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Rabbit polyclonal to NSL1
Predicted to work with:
Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between C terminal amino acids 230-281 of Human NSL1 (NP_056286.2)
Whole cell lysate from HeLa and 293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
Information by UniProt
Immunoprecipitation - Anti-NSL1 antibody (ab70836)
Detection of Human NSL1 by Western Blot of
Immunoprecipitates in Whole cell lysate (1 mg for IP,
20% of IP loaded) from HeLa cells.
used for IP at 3 µg/mg lysate.
Lane 2: immunoprecipitation with an other antibody
Lane 3: ab70836 used for IP at 3 µg/mg lysate
Lane 4: Control IgG IP
For blotting ab70836 was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
Western blot - Anti-NSL1 antibody (ab70836)
All lanes :
Anti-NSL1 antibody (ab70836) at 0.1 µg/ml
Lane 1 :
Whole cell lysate from HeLa cells at 50 µg
Lane 2 :
Whole cell lysate from HeLa cells at 15 µg
Lane 3 :
Whole cell lysate from HeLa cells at 5 µg
Lane 4 :
Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
38 kDa (
why is the actual band size different from the predicted?
Additional bands at:
28 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"