Overview

  • Product name
  • Description
    Rabbit polyclonal to NSL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between C terminal amino acids 230-281 of Human NSL1 (NP_056286.2)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).

Target

  • Function
    Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4833432M17Rik antibody
    • AI451597 antibody
    • C1orf48 antibody
    • DC31 antibody
    • DC8 antibody
    • DKFZp566O1646 antibody
    • Gm105 antibody
    • Gm984 antibody
    • Kinetochore associated protein NSL1 homolog antibody
    • Kinetochore-associated protein NSL1 homolog antibody
    • MIS14 antibody
    • NSL1 antibody
    • NSL1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody
    • NSL1_HUMAN antibody
    see all

Images

  • Detection of Human NSL1 by Western Blot of Immunoprecipitates in Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
    Lane 1: ab70835 used for IP at 3 µg/mg lysate.
    Lane 2: immunoprecipitation with an other antibody
    Lane 3: ab70836 used for IP at 3 µg/mg lysate
    Lane 4: Control IgG IP
    For blotting ab70836 was used at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 3 minutes.
  • All lanes : Anti-NSL1 antibody (ab70836) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 32 kDa
    Observed band size: 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes

References

ab70836 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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