Key features and details
- Rabbit polyclonal to NSMAF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NSMAF antibody
See all NSMAF primary antibodies
DescriptionRabbit polyclonal to NSMAF
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide derived from human NSMAF protein.
- Human brain lysate, RMS-13 rhabdosarcoma cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab81260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 104 kDa.|
RelevanceNSMAF is a novel adaptor protein which mediates the activation of sphingomyelinase by receptors such as tumor necrosis factor receptor type 1 and CD40 and subsequent apoptosis in cardiomyocytes. Inhibition of this factor may provide a novel intervention point for reducing ischemia/reperfusion injury.
- Factor associated with N SMase activation antibody
- Factor associated with neutral sphingomyelinase antibody
- Factor associated with neutral sphingomyelinase activation antibody
All lanes : Anti-NSMAF antibody (ab81260) at 1/400 dilution
Lane 1 : human
Lane 2 : RMS-13
rhabdosarcoma cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : mouse anti-rabbit HRP at 1/50000 dilution
Predicted band size: 104 kDa
Observed band size: 104 kDa
Exposure time: 5 minutes
Visualized using Pierce West Femto substrate system.
ab81260 has been referenced in 1 publication.
- Leidal AM et al. The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Nat Cell Biol 22:187-199 (2020). PubMed: 31932738