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    nsmaf-antibody-ab96804.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Anti-NSMAF antibody (ab96804)

  • Datasheet
  • SDS
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Western blot - Anti-NSMAF antibody (ab96804)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMAF antibody (ab96804)

Key features and details

  • Rabbit polyclonal to NSMAF
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-NSMAF antibody
    See all NSMAF primary antibodies
  • Description

    Rabbit polyclonal to NSMAF
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 262 - 540 of Human NSMAF (NP_003571).

  • Positive control

    • WB: 293T cell lysate IHC-P: T(gastric CA)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 104 kDa.
IHC-P
1/100.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 104 kDa.
IHC-P
1/100.

Target

  • Relevance

    NSMAF is a novel adaptor protein which mediates the activation of sphingomyelinase by receptors such as tumor necrosis factor receptor type 1 and CD40 and subsequent apoptosis in cardiomyocytes. Inhibition of this factor may provide a novel intervention point for reducing ischemia/reperfusion injury.
  • Database links

    • Entrez Gene: 8439 Human
    • Entrez Gene: 18201 Mouse
    • Entrez Gene: 353233 Rat
    • Omim: 603043 Human
    • SwissProt: Q92636 Human
    • SwissProt: O35242 Mouse
    • SwissProt: Q7TSY5 Rat
    • Unigene: 372000 Human
    • Alternative names

      • Factor associated with N SMase activation antibody
      • Factor associated with neutral sphingomyelinase antibody
      • Factor associated with neutral sphingomyelinase activation antibody
      • FAN antibody
      • Neutral sphingomyelinase (N SMase) activation associated factor antibody
      • Protein FAN antibody
      see all

    Images

    • Western blot - Anti-NSMAF antibody (ab96804)
      Western blot - Anti-NSMAF antibody (ab96804)
      All lanes : Anti-NSMAF antibody (ab96804) at 1/500 dilution

      Lane 1 : Non-transfected 293T cell lysate
      Lane 2 : NSMAF transfected 293T cell lysate

      Predicted band size: 104 kDa



      7.5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMAF antibody (ab96804)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMAF antibody (ab96804)
      ab96804, at a 1/100 dilution, staining NSMAF in paraffin-embedded T(gastric CA) by Immunohistochemical analysis.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab96804? Please let us know so that we can cite the reference in this datasheet.

    ab96804 has not yet been referenced specifically in any publications.

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