Recombinant Anti-nSMase antibody [EPR6718] (ab131330)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6718] to nSMase
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-nSMase antibody [EPR6718]
See all nSMase primary antibodies -
Description
Rabbit monoclonal [EPR6718] to nSMase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human nSMase aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: 293T, MOLT4 and K562 cell lysates; U-87MG cells; Mouse brain and Rat brain lysates. IP: Mouse brain lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6718 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa). |
IP
1/10 - 1/100. |
Target
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Function
Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. -
Sequence similarities
Belongs to the neutral sphingomyelinase family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6610 Human
- Entrez Gene: 20598 Mouse
- Entrez Gene: 83537 Rat
- Omim: 603498 Human
- SwissProt: O60906 Human
- SwissProt: O70572 Mouse
- SwissProt: Q8BW85 Mouse
- SwissProt: Q8C6H6 Mouse
see all -
Alternative names
- ISC1 antibody
- Lyso platelet activating factor phospholipase C antibody
- Lyso-PAF-PLC antibody
see all
Images
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ab131330 (purified) at 1:30 dilution (2µg) immunoprecipitating nSMase in Mouse brain lysate.
Lane 1 (input): Mouse brain lysate 10µg
Lane 2 (+): ab131330 & Mouse brain lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab131330 in Mouse brain lysate
For western blotting, VeriBlot for IP Detection Reagent(HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution (Purified)
Lane 1 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa -
All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDaThis image was generated using the unpurified version of the product.
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All lanes : Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDaThis image was generated using the unpurified version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab131330 has been referenced in 5 publications.
- Liu XT et al. Ablation of sphingosine kinase 2 suppresses fatty liver-associated hepatocellular carcinoma via downregulation of ceramide transfer protein. Oncogenesis 11:67 (2022). PubMed: 36333295
- Dowdy T et al. Sphingolipid Pathway as a Source of Vulnerability in IDH1mut Glioma. Cancers (Basel) 12:N/A (2020). PubMed: 33050528
- Petrov AM et al. Changes in Membrane Ceramide Pools in Rat Soleus Muscle in Response to Short-Term Disuse. Int J Mol Sci 20:N/A (2019). PubMed: 31574943
- Sen NE et al. In Human and Mouse Spino-Cerebellar Tissue, Ataxin-2 Expansion Affects Ceramide-Sphingomyelin Metabolism. Int J Mol Sci 20:N/A (2019). PubMed: 31766565
- Russ DW et al. Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism. Biogerontology 16:747-59 (2015). WB ; Rat . PubMed: 26296420