Overview

  • Product name

    Anti-nSMase antibody [EPR6718] - BSA and Azide free
    See all nSMase primary antibodies
  • Description

    Rabbit monoclonal [EPR6718] to nSMase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human nSMase aa 1-100. The exact sequence is proprietary.

  • Positive control

    • 293T, MOLT4 and K562 cell lysates; U-87MG cells; Mouse brain and Rat brain lysates.
  • General notes

    Ab243913 is the carrier-free version of ab131330. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab243913 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab243913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    Images

    • ab131330 (purified) at 1:30 dilution (2µg) immunoprecipitating nSMase in Mouse brain lysate.
      Lane 1 (input): Mouse brain lysate 10µg
      Lane 2 (+): ab131330 & Mouse brain lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab131330 in Mouse brain lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    • Equilibrium disassociation constant (KD)
      Learn more about K

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131330).

    References

    ab243913 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab243913.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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