Overview

  • Product name

  • Description

    Mouse polyclonal to NSMase2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length human SMPD3 protein (NP_061137.1).

  • Positive control

    • nSMase2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 71 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.
  • Tissue specificity

    Predominantly expressed in brain.
  • Sequence similarities

    Belongs to the neutral sphingomyelinase family.
  • Developmental stage

    Up-regulated during G0/G1 phases.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons. According to PubMed:15051724, it localizes to plasma membrane in confluent contact-inhibited cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cca1 antibody
    • Confluent 3Y1 cell-associated protein 1 antibody
    • Neutral sphingomyelinase 2 antibody
    • Neutral sphingomyelinase II antibody
    • NSMA2_HUMAN antibody
    • nSMase-2 antibody
    • nSMase2 antibody
    • SMPD3 antibody
    • Sphingomyelin phosphodiesterase 3 antibody
    • sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) antibody
    see all

Images

  • All lanes : Anti-NSMase2 antibody (ab68735) at 1/500 dilution

    Lane 1 : nSMase2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 71 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?

  • IHC image of ab68735 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68735, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Im EJ  et al. Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A. Nat Commun 10:1387 (2019). Read more (PubMed: 30918259) »
See 1 Publication for this product

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