Key features and details
- Mouse polyclonal to NSMCE1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NSMCE1 antibody
See all NSMCE1 primary antibodies
DescriptionMouse polyclonal to NSMCE1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Cow, Dog, Rhesus monkey, Orangutan
- Lysate from 293T cells transfected with NSMCE1; HeLa cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab168578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
RelevanceNSMCE1 is a probable component of the SMC5-SMC6 complex, a complex involved in DNA double strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double strand breaks.
Cellular localizationNucleus. Chromosome, telomere
- HSPC333 antibody
- HSPC337 antibody
- Non SMC element 1 homolog (S. cerevisiae) antibody
All lanes : Anti-NSMCE1 antibody (ab168578) at 1 µg/ml
Lane 1 : NSMCE1-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG
Developed using the ECL technique.
Predicted band size: 30 kDa
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling NSMCE1 using ab168578 at 10 µg/ml.
ab168578 has not yet been referenced specifically in any publications.