RecombinantRabMAb

Recombinant Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
  • Western blot - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
  • Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9103(B)] to NSMCE1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NSMCE1 antibody [EPR9103(B)]
    See all NSMCE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9103(B)] to NSMCE1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NSMCE1 aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human spleen and cervical carcinoma tissues; Raji, HepG2, Jurkat and HL60 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9103(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Relevance

      NSMCE1 is a probable component of the SMC5-SMC6 complex, a complex involved in DNA double strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double strand breaks.

    • Cellular localization

      Nucleus. Chromosome, telomere

    • Database links

      see all

    • Alternative names

      • HSPC333 antibody
      • HSPC337 antibody
      • Non SMC element 1 homolog (S. cerevisiae) antibody
      • Non SMC element 1 homolog antibody
      • Non structural maintenance of chromosomes element 1 homolog antibody
      • NSE1 antibody
      • NSMCE 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling NSMCE1 with ab139689 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling NSMCE1 with ab139689 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
      Western blot - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
      All lanes : Anti-NSMCE1 antibody [EPR9103(B)] (ab139689) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HL60 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    • Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
      Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

    References (1)

    Publishing research using ab139689? Please let us know so that we can cite the reference in this datasheet.

    ab139689 has been referenced in 1 publication.

    • Xu W  et al. PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. J Virol 92:N/A (2018). PubMed: 30185588

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab139689.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com