Recombinant
RabMAb

Recombinant Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

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Overview

  • Product name

    Anti-NSMCE1 antibody [EPR9103(B)]
    See all NSMCE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9103(B)] to NSMCE1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NSMCE1 aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human spleen and cervical carcinoma tissues; Raji, HepG2, Jurkat and HL60 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9103(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab139689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Relevance

      NSMCE1 is a probable component of the SMC5-SMC6 complex, a complex involved in DNA double strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double strand breaks.

    • Cellular localization

      Nucleus. Chromosome, telomere

    • Database links

      see all

    • Alternative names

      • HSPC333 antibody
      • HSPC337 antibody
      • Non SMC element 1 homolog (S. cerevisiae) antibody
      • Non SMC element 1 homolog antibody
      • Non structural maintenance of chromosomes element 1 homolog antibody
      • NSE1 antibody
      • NSMCE 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling NSMCE1 with ab139689 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling NSMCE1 with ab139689 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
      Western blot - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
      All lanes : Anti-NSMCE1 antibody [EPR9103(B)] (ab139689) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HL60 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    References

    This product has been referenced in:

    • Xu W  et al. PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. J Virol 92:N/A (2018). Read more (PubMed: 30185588) »
    See 1 Publication for this product

    Publishing research using ab139689? Please let us know so that we can cite the reference in this datasheet.

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