Name: Anti-NSMCE1 antibody [EPR9103(B)]
Brand: RabMAb
SKU: ab139689

Product name

Anti-NSMCE1 antibody [EPR9103(B)]
See all NSMCE1 primary antibodies
Description

Rabbit monoclonal [EPR9103(B)] to NSMCE1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human NSMCE1 aa 250-350 (C terminal). The exact sequence is proprietary.
Positive control
- Human spleen and cervical carcinoma tissues; Raji, HepG2, Jurkat and HL60 cell lysates.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9103(B)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab139689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P		1/50 - 1/100.
IP		1/10 - 1/100.

Application notes

Is unsuitable for Flow Cyt or ICC.

Relevance

NSMCE1 is a probable component of the SMC5-SMC6 complex, a complex involved in DNA double strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double strand breaks.
Cellular localization

Nucleus. Chromosome, telomere
Database links
- Entrez Gene: 197370 Human
- Entrez Gene: 67711 Mouse
- Entrez Gene: 361645 Rat
- SwissProt: Q8WV22 Human
- SwissProt: Q9D720 Mouse
- SwissProt: Q499U6 Rat
- Unigene: 284295 Human
- Unigene: 4467 Mouse
- Unigene: 17063 Rat
see all
Alternative names
- HSPC333 antibody
- HSPC337 antibody
- Non SMC element 1 homolog (S. cerevisiae) antibody
- Non SMC element 1 homolog antibody
- Non structural maintenance of chromosomes element 1 homolog antibody
- NSE1 antibody
- NSMCE 1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling NSMCE1 with ab139689 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling NSMCE1 with ab139689 at 1/50 dilution.
Western blot - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

All lanes : Anti-NSMCE1 antibody [EPR9103(B)] (ab139689) at 1/1000 dilution

Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HL60 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 31 kDa

Xu W et al. PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. J Virol 92:N/A (2018). Read more (PubMed: 30185588) »

Publishing research using ab139689? Please let us know so that we can cite the reference in this datasheet.

Recombinant Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)

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