Recombinant Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9103(B)] to NSMCE1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-NSMCE1 antibody [EPR9103(B)]
See all NSMCE1 primary antibodies -
Description
Rabbit monoclonal [EPR9103(B)] to NSMCE1 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human NSMCE1 aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- Human spleen and cervical carcinoma tissues; Raji, HepG2, Jurkat and HL60 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9103(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
NSMCE1 is a probable component of the SMC5-SMC6 complex, a complex involved in DNA double strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double strand breaks. -
Cellular localization
Nucleus. Chromosome, telomere -
Database links
- Entrez Gene: 197370 Human
- Entrez Gene: 67711 Mouse
- Entrez Gene: 361645 Rat
- SwissProt: Q8WV22 Human
- SwissProt: Q9D720 Mouse
- SwissProt: Q499U6 Rat
- Unigene: 284295 Human
- Unigene: 4467 Mouse
see all -
Alternative names
- HSPC333 antibody
- HSPC337 antibody
- Non SMC element 1 homolog (S. cerevisiae) antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling NSMCE1 with ab139689 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling NSMCE1 with ab139689 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-NSMCE1 antibody [EPR9103(B)] (ab139689) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab139689 has been referenced in 1 publication.
- Xu W et al. PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. J Virol 92:N/A (2018). PubMed: 30185588