Recombinant
Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-NSMCE1 antibody [EPR9103(B)]
See all NSMCE1 primary antibodies -
DescriptionRabbit monoclonal [EPR9103(B)] to NSMCE1
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human NSMCE1 aa 250-350 (C terminal). The exact sequence is proprietary.
-
Positive control
- Human spleen and cervical carcinoma tissues; Raji, HepG2, Jurkat and HL60 cell lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20ºC.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR9103(B)
-
IsotypeIgG
-
Research areas
Associated products
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab139689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 31 kDa. | |
| IHC-P | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt or ICC.
Target
-
RelevanceNSMCE1 is a probable component of the SMC5-SMC6 complex, a complex involved in DNA double strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double strand breaks.
-
Cellular localizationNucleus. Chromosome, telomere
-
Database links
- Entrez Gene: 197370 Human
- Entrez Gene: 67711 Mouse
- Entrez Gene: 361645 Rat
- SwissProt: Q8WV22 Human
- SwissProt: Q9D720 Mouse
- SwissProt: Q499U6 Rat
- Unigene: 284295 Human
- Unigene: 4467 Mouse
see all -
Alternative names
- HSPC333 antibody
- HSPC337 antibody
- Non SMC element 1 homolog (S. cerevisiae) antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)](https://www.abcam.com/ps/datasheet/images/139/ab139689/NSMCE1-Primary-antibodies-ab139689-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling NSMCE1 with ab139689 at 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)](https://www.abcam.com/ps/datasheet/images/139/ab139689/NSMCE1-Primary-antibodies-ab139689-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling NSMCE1 with ab139689 at 1/50 dilution. -
![Western blot - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)](https://www.abcam.com/ps/datasheet/images/139/ab139689/NSMCE1-Primary-antibodies-ab139689-3.jpg)
Western blot - Anti-NSMCE1 antibody [EPR9103(B)] (ab139689)All lanes : Anti-NSMCE1 antibody [EPR9103(B)] (ab139689) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Datasheets and documents
References
ab139689 has not yet been referenced specifically in any publications.
