Key features and details
- Rabbit polyclonal to NSP 5 alpha 3 alpha
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NSP 5 alpha 3 alpha antibody
See all NSP 5 alpha 3 alpha primary antibodies
DescriptionRabbit polyclonal to NSP 5 alpha 3 alpha
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residues 450 and 500 of human NSP 5 alpha 3 alpha (NP_001028725.1).
- Whole cell lysate from HeLa cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab87801 was affinity purified using an epitope specific to NSP 5 alpha 3 alpha immobilized on solid support.
Our Abpromise guarantee covers the use of ab87801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 119 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityHighly expressed in testis. Barely detectable in other tissues. Also highly expressed in some cancer cell lines.
Involvement in diseaseNote=A chromosomal aberration involving CYTSB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with PDGFRB.
Sequence similaritiesBelongs to the cytospin-A family.
Contains 1 CH (calponin-homology) domain.
- Information by UniProt
- Cytokinesis and spindle organization B antibody
- Cytospin B antibody
- Cytospin-B antibody
All lanes : Anti-NSP 5 alpha 3 alpha antibody (ab87801) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 119 kDa
Observed band size: 119 kDa
Exposure time: 30 seconds
Detection of NSP 5 alpha 3 alpha in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87801 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab87801 has not yet been referenced specifically in any publications.