Anti-NSP1 antibody [32D6] (ab4641)
![Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)](https://www.abcam.com/ps/products/4/ab4641/Images/Nsp1p-Primary-antibodies-ab4641-5.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)")
Key features and details
- Mouse monoclonal [32D6] to NSP1
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-NSP1 antibody [32D6] -
Description
Mouse monoclonal [32D6] to NSP1 -
Host species
Mouse -
Specificity
This antibody was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert bearing ~66% of the C-terminal portion of NSP1. To confirm that 32D6 possessed high affinity for NSP1, strain RS453, which expresses a shortened isoform of NSP1, was compared to the wildtype strain BJ5465. NSP1 in RS453 contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for function and encodes a protein that has been observed to migrate at approxmately 85 kDa on SDS gels. The predicted size of wildtype NSP1 is 86.5 kDa, but NSP1 has been observed to migrate on SDS gels at ~100 kDa. In our gels, the apparent molecular mass of wildtype NSP1 was 108 kDa, whereas the short isoform of NSP1 in RS453 migrated at 91 kDa. The detection of two protein bands of apparent sizes 108 kDa and 91 kDa in BJ5465 and RS453, respectively, demonstrated that 32D6 recognized the pore complex protein NSP1. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.
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General notes
This antibody is a useful marker of yeast nuclear pores.
Previously labelled as Nsp1p.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Sterile filtered. -
Primary antibody notes
This antibody is a useful marker of yeast nuclear pores. -
Clonality
Monoclonal -
Clone number
32D6 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/1000.
Yeast cells. |
| WB |
1/10000 - 1/50000. Detects a band of approximately 108,91 kDa (predicted molecular weight: 85 kDa).
1/10000 (cell lysates) - 1/50000 (nuclear fractions). For other (non-ECL) detection methods, 1/1000 - 1/5000. |
| Notes |
|---|
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ICC/IF
1/1000. Yeast cells. |
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WB
1/10000 - 1/50000. Detects a band of approximately 108,91 kDa (predicted molecular weight: 85 kDa). 1/10000 (cell lysates) - 1/50000 (nuclear fractions). For other (non-ECL) detection methods, 1/1000 - 1/5000. |
Target
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Relevance
NSP1 functions as a component of the nuclear pore complex (NPC). It plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, pre-ribosome, signal recognition particle (SRP), and protein transport. -
Cellular localization
Nuclear membrane: part of the nuclear pore complex, it presents a symmetric distribution with a cytoplasmic and a nucleoplasmic side. -
Database links
- Entrez Gene: 853409 Saccharomyces cerevisiae
- SwissProt: P14907 Saccharomyces cerevisiae
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Alternative names
- NSP1 antibody
- Nsp1p antibody
- Nuclear pore protein NSP1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)Image courtesy of an anonymous Abreview.
ab4641 (1/5000) detecting NSP1 in Saccharomyces cerevisae DDY1810 (green). Cells were fixed in paraformaldehyde, permeabilized in methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue). For further experimental details please refer to Abreview.
Protocols
Datasheets and documents
References (7)
ab4641 has been referenced in 7 publications.
- Bittmann J et al. An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases. Elife 9:N/A (2020). PubMed: 32352375
- Zhang W et al. Brr6 and Brl1 locate to nuclear pore complex assembly sites to promote their biogenesis. J Cell Biol 217:877-894 (2018). PubMed: 29439116
- Rüthnick D et al. Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes. J Cell Biol 216:2425-2442 (2017). PubMed: 28659328
- Colombi P et al. The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1. J Cell Biol 203:215-32 (2013). WB, ICC/IF . PubMed: 24165936
- Ringel AE et al. Yeast Tdh3 (glyceraldehyde 3-phosphate dehydrogenase) is a Sir2-interacting factor that regulates transcriptional silencing and rDNA recombination. PLoS Genet 9:e1003871 (2013). ICC/IF ; Saccharomyces cerevisiae . PubMed: 24146631
- Beach A et al. Mitochondrial membrane lipidome defines yeast longevity. Aging (Albany NY) 5:551-74 (2013). PubMed: 23924582
- Motwani T et al. Sir3 and epigenetic inheritance of silent chromatin in Saccharomyces cerevisiae. Mol Cell Biol 32:2784-93 (2012). ICC/IF ; Saccharomyces cerevisiae . PubMed: 22586263
