Anti-NSP1 antibody [32D6] (ab4641)

Reviews (1)Q&A (1)References (5)

Overview

  • Product name
    Anti-NSP1 antibody [32D6]
  • Description
    Mouse monoclonal [32D6] to NSP1
  • Host species
    Mouse
  • Specificity
    This antibody was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert bearing ~66% of the C-terminal portion of NSP1. To confirm that 32D6 possessed high affinity for NSP1, strain RS453, which expresses a shortened isoform of NSP1, was compared to the wildtype strain BJ5465. NSP1 in RS453 contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for function and encodes a protein that has been observed to migrate at approxmately 85 kDa on SDS gels. The predicted size of wildtype NSP1 is 86.5 kDa, but NSP1 has been observed to migrate on SDS gels at ~100 kDa. In our gels, the apparent molecular mass of wildtype NSP1 was 108 kDa, whereas the short isoform of NSP1 in RS453 migrated at 91 kDa. The detection of two protein bands of apparent sizes 108 kDa and 91 kDa in BJ5465 and RS453, respectively, demonstrated that 32D6 recognized the pore complex protein NSP1.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

  • General notes


    This antibody is a useful marker of yeast nuclear pores.

    Previously labelled as Nsp1p.

Properties

Applications

Our Abpromise guarantee covers the use of ab4641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

Yeast cells.
WB 1/10000 - 1/50000. Detects a band of approximately 108,91 kDa (predicted molecular weight: 85 kDa).

1/10000 (cell lysates) - 1/50000 (nuclear fractions). For other (non-ECL) detection methods, 1/1000 - 1/5000.

Target

  • Relevance
    NSP1 functions as a component of the nuclear pore complex (NPC). It plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, pre-ribosome, signal recognition particle (SRP), and protein transport.
  • Cellular localization
    Nuclear membrane: part of the nuclear pore complex, it presents a symmetric distribution with a cytoplasmic and a nucleoplasmic side.
  • Database links
    • Alternative names
      • NSP1 antibody
      • Nsp1p antibody
      • Nuclear pore protein NSP1 antibody
      • Nucleoporin NSP1 antibody
      • p110 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)
      Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)Image courtesy of an anonymous Abreview.

      ab4641 (1/5000) detecting NSP1 in Saccharomyces cerevisae DDY1810 (green). Cells were fixed in paraformaldehyde, permeabilized in methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue). For further experimental details please refer to Abreview.

      See Abreview

    References

    This product has been referenced in:
    • Rüthnick D  et al. Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes. J Cell Biol 216:2425-2442 (2017). Read more (PubMed: 28659328) »
    • Colombi P  et al. The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1. J Cell Biol 203:215-32 (2013). WB, ICC/IF . Read more (PubMed: 24165936) »
    See all 5 Publications for this product

    Publishing research using ab4641? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NSP1 antibody [32D6]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Saccharomyces cerevisiae Cell (Yeast cells DDY1810)
    Permeabilization
    Yes - methanol/acetone
    Specification
    Yeast cells DDY1810
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50µg/mL · Temperature: RT°C
    Fixative
    Paraformaldehyde
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    Abcam user community

    Verified customer

    Submitted Sep 28 2010

    Question

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    Answer

    Thank you for your enquiry. I have contacted the source of the three antibodies and as such unfortunately I cannot give you an exact figure on the protein or IgG concentration of the antibodies. However, all three are derived from tissue culture supernatants and are therefore in the range of 0.5mgml-1 to 1.0mgml-1. Please do not hesitate to contact me should you require further assistance.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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