Immunocytochemistry/ Immunofluorescence abreview for Anti-NSP1 antibody [32D6]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Saccharomyces cerevisiae Cell (Yeast cells DDY1810)
Permeabilization
Yes - methanol/acetone
Specification
Yeast cells DDY1810
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50µg/mL · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA in PBS
Dilution
1/5000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: alexa fluor 488 donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

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Submitted Sep 28 2010

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