Overview

  • Product name

  • Description

    Rabbit polyclonal to NSP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 485-677 of Human NSP3

  • Positive control

    • SK-N-SH whole cell lysate, Human hepatoma tissue, Mouse muscle tissue
  • General notes

    Keep as a concentrated solution

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 94 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Eph receptor-binding protein which may be a positive regulator of TCR signaling. Binding to BCAR1 is required to induce membrane ruffling and promote EGF-dependent cell migration.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Contains 1 Ras-GEF domain.
    Contains 1 SH2 domain.
  • Cellular localization

    Cytoplasm. Membrane. Associated with the membrane when EGF-stimulated. Found at ruffling membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cas/HEF1 associated signal transducer antibody
    • CHAT antibody
    • FLJ39664 antibody
    • Novel SH2 containing protein 3 antibody
    • Novel SH2-containing protein 3 antibody
    • NSP 3 antibody
    • PRO34088 antibody
    • SH2 domain containing 3C antibody
    • SH2 domain containing Eph receptor binding protein 1 antibody
    • SH2 domain containing protein 3C antibody
    • SH2 domain-containing protein 3C antibody
    • SH2D3_HUMAN antibody
    • SH2D3C antibody
    • Shep1 antibody
    see all

Images

  • Anti-NSP3 antibody (ab126130) at 1/500 dilution + Sk-N-SH whole cell lysate at 30 µg

    Predicted band size: 94 kDa

  • ab126130, at 1/100 dilution, staining paraffin embedded Human hepatoma by immunohistochemistry.
  • ab126130, at 1/100 dilution, staining paraffin embedded Mouse muscle by immunohistochemistry.

References

ab126130 has not yet been referenced specifically in any publications.

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