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    nsr1p-antibody-31c4-ab4642.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Nucleolus
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Anti-Nsr1p antibody [31C4] (ab4642)

  • Datasheet
Reviews (2) Submit a question References (1)

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Western blot - Anti-Nsr1p antibody [31C4] (ab4642)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)

Key features and details

  • Mouse monoclonal [31C4] to Nsr1p
  • Suitable for: ICC/IF, WB
  • Reacts with: Saccharomyces cerevisiae
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-Nsr1p antibody [31C4]
  • Description

    Mouse monoclonal [31C4] to Nsr1p
  • Host species

    Mouse
  • Specificity

    This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.065% Sodium azide
    Constituent: Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Purification notes

    Sterile filtered.
  • Clonality

    Monoclonal
  • Clone number

    31C4
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleolus
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/500 - 1/5000. (yeast cells).
WB (1)
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.
Notes
ICC/IF
1/500 - 1/5000. (yeast cells).
WB
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.

Target

  • Relevance

    Nsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 853064 Saccharomyces cerevisiae
    • SwissProt: P27476 Saccharomyces cerevisiae
    • Alternative names

      • NSR1 antibody
      • Nuclear localization sequence binding protein antibody
      • p67 antibody
      • SHE5 antibody

    Images

    • Western blot - Anti-Nsr1p antibody [31C4] (ab4642)
      Western blot - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
      All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution

      Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
      Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate

      Secondary
      All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution

      Performed under reducing conditions.

      Predicted band size: 45 kDa



      Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000)

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)
      Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
      ab4642 (1/5000) detecting Nsr1p in DDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
      Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
      For further experimental details please refer to Abreview.

      See Abreview

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab4642? Please let us know so that we can cite the reference in this datasheet.

    ab4642 has been referenced in 1 publication.

    • Lista MJ  et al. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat Commun 8:16043 (2017). WB . PubMed: 28685753

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nsr1p antibody [31C4] - Nucleolar Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Saccharomyces cerevisiae Cell (Yeast cells DDY1810)
    Specification
    Yeast cells DDY1810
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - methanol/acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 28 2010

    Western blot abreview for Anti-Nsr1p antibody [31C4] - Nucleolar Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (DDY1810)
    Loading amount
    0.5 µg
    Specification
    DDY1810
    Gel Running Conditions
    Reduced Denaturing (4-12% NUPage gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 28 2010

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