Anti-Nsr1p antibody [31C4] (ab4642)
![Western blot - Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.com/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-5.jpg "Western blot - Anti-Nsr1p antibody [31C4] (ab4642)")
Key features and details
- Mouse monoclonal [31C4] to Nsr1p
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Nsr1p antibody [31C4] -
Description
Mouse monoclonal [31C4] to Nsr1p -
Host species
Mouse -
Specificity
This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Tissue, cells or virus corresponding to Nsr1p. Yeast nuclear preparation
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Sterile filtered. -
Clonality
Monoclonal -
Clone number
31C4 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/500 - 1/5000. (yeast cells).
|
| WB | (1) |
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.
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| Notes |
|---|
|
ICC/IF
1/500 - 1/5000. (yeast cells). |
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WB
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000. |
Target
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Relevance
Nsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 853064 Saccharomyces cerevisiae
- SwissProt: P27476 Saccharomyces cerevisiae
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Alternative names
- NSR1 antibody
- Nuclear localization sequence binding protein antibody
- p67 antibody
- SHE5 antibody
Images
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Western blot - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution
Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate
Secondary
All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000) -
![Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.com/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.ab4642 (1/5000) detecting Nsr1p inDDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
For further experimental details please refer to Abreview.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab4642 has been referenced in 2 publications.
- Azevedo C et al. Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation. J Biol Chem 295:1439-1451 (2020). PubMed: 31844018
- Lista MJ et al. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat Commun 8:16043 (2017). WB . PubMed: 28685753
