Anti-Nsr1p antibody [31C4] (ab4642)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Nsr1p antibody [31C4]
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DescriptionMouse monoclonal [31C4] to Nsr1p
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Host speciesMouse
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SpecificityThis antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p.
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Saccharomyces cerevisiae
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Immunogen
This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
PurityTissue culture supernatant
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Purification notesSterile filtered.
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ClonalityMonoclonal
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Clone number31C4
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab4642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500 - 1/5000. (yeast cells). | |
| WB | 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000. |
Target
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RelevanceNsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 853064 Saccharomyces cerevisiae
- SwissProt: P27476 Saccharomyces cerevisiae
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Alternative names
- NSR1 antibody
- Nuclear localization sequence binding protein antibody
- p67 antibody
- SHE5 antibody
Images
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![Western blot - Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.com/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-5.jpg)
Western blot - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution
Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate
Secondary
All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000) -
![Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.com/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.ab4642 (1/5000) detecting Nsr1p inDDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
For further experimental details please refer to Abreview.
Datasheets and documents
References
This product has been referenced in:
- Lista MJ et al. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat Commun 8:16043 (2017). WB . Read more (PubMed: 28685753) »
