Anti-Nsr1p antibody [31C4] (ab4642)

Reviews (2) Submit a question References (1)
Western blot - Anti-Nsr1p antibody [31C4] (ab4642)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)

Key features and details

  • Mouse monoclonal [31C4] to Nsr1p
  • Suitable for: ICC/IF, WB
  • Reacts with: Saccharomyces cerevisiae
  • Isotype: IgG

Overview

  • Product name

    Anti-Nsr1p antibody [31C4]

  • Description

    Mouse monoclonal [31C4] to Nsr1p

  • Host species

    Mouse

  • Specificity

    This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p.

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Saccharomyces cerevisiae

  • Immunogen

    This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/500 - 1/5000. (yeast cells).
WB (1)
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.
Notes
ICC/IF
1/500 - 1/5000. (yeast cells).
WB
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.

Target

  • Relevance

    Nsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • NSR1 antibody
      • Nuclear localization sequence binding protein antibody
      • p67 antibody
      • SHE5 antibody

    Images

    • Western blot - Anti-Nsr1p antibody [31C4] (ab4642)
      Western blot - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
      All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution

      Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
      Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate

      Secondary
      All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution

      Performed under reducing conditions.

      Predicted band size: 45 kDa



      Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000)

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)
      Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
      ab4642 (1/5000) detecting Nsr1p in DDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
      Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
      For further experimental details please refer to Abreview.

      See Abreview

    References (1)

    Publishing research using ab4642? Please let us know so that we can cite the reference in this datasheet.

    ab4642 has been referenced in 1 publication.

    • Lista MJ  et al. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat Commun 8:16043 (2017). WB . PubMed: 28685753

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nsr1p antibody [31C4] - Nucleolar Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Saccharomyces cerevisiae Cell (Yeast cells DDY1810)
    Specification
    Yeast cells DDY1810
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - methanol/acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 28 2010

    Western blot abreview for Anti-Nsr1p antibody [31C4] - Nucleolar Marker

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (DDY1810)
    Loading amount
    0.5 µg
    Specification
    DDY1810
    Gel Running Conditions
    Reduced Denaturing (4-12% NUPage gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 28 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com