Product nameAnti-NSRP1 antibody
DescriptionRabbit polyclonal to NSRP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- HeLa, 293T, and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab200567 was affinity purified using an epitope specific to NSRP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab200567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the CCDC55 family.
- Information by UniProt
- CCD55_HUMAN antibody
- Ccdc55 antibody
- Coiled coil domain containing 55 antibody
All lanes : Anti-NSRP1 antibody (ab200567) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 66 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
Detection of NSRP1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab200567 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab200567 has not yet been referenced specifically in any publications.