Anti-NSUN2/SAKI antibody (ab128243)
Key features and details
- Rabbit polyclonal to NSUN2/SAKI
- Suitable for: ELISA, WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-NSUN2/SAKI antibody
See all NSUN2/SAKI primary antibodies -
Description
Rabbit polyclonal to NSUN2/SAKI -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse NSUN2/SAKI (internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Constituents: 1.2% Tris, 0.85% Glycine, 2% Sucrose, 95.95% Water -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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|
WB |
1/1000 - 1/5000.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/1000 - 1/5000. |
Target
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Function
RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. -
Sequence similarities
Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. -
Post-translational
modificationsPhosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 54888 Human
- Entrez Gene: 28114 Mouse
- Entrez Gene: 361191 Rat
- Omim: 610916 Human
- SwissProt: Q08J23 Human
- SwissProt: Q1HFZ0 Mouse
- Unigene: 481526 Human
- Unigene: 260009 Mouse
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Alternative names
- 5 methycytoisine methyltransferase antibody
- D13Wsu123e antibody
- FLJ20303 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab128243 has not yet been referenced specifically in any publications.