Key features and details
- Rabbit polyclonal to NSUN2/SAKI
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NSUN2/SAKI antibody
See all NSUN2/SAKI primary antibodies
DescriptionRabbit polyclonal to NSUN2/SAKI
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
- HeLa cell lysate.
This product was previously labelled as NSUN2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
FunctionRNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation.
Sequence similaritiesBelongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.
modificationsPhosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.
Cellular localizationNucleus > nucleolus. Cytoplasm. Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis.
- Information by UniProt
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ab75493 has not yet been referenced specifically in any publications.