Overview

  • Product name

  • Description

    Rabbit polyclonal to NSUN5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: QLPRFVRVNT LKTCSDDVVD YFKRQGFSYQ GRASSLDDLR ALKGKHFLLD PLMPELLVFP AQTDLHEHPL YRAGHLIL, corresponding to amino acids 133-210 of Human NSUN5.

  • Positive control

    • U 251 MG lysate; Human rectum tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function

    May have S-adenosyl-L-methionine-dependent methyl-transferase activity.
  • Tissue specificity

    Ubiquitous. Detected in placenta, heart and skeletal muscle.
  • Involvement in disease

    Note=NSUN5 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities

    Belongs to the methyltransferase superfamily. RsmB/NOP family.
  • Post-translational
    modifications

    Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ10267 antibody
    • MGC986 antibody
    • NOL1 antibody
    • NOL1-related protein antibody
    • NOL1/NOP2/Sun domain family member 5 antibody
    • NOL1R antibody
    • NOP2/Sun domain family, member 5 antibody
    • NOP2/Sun domain family, member 5A antibody
    • NSUN5 antibody
    • NSUN5_HUMAN antibody
    • p120 antibody
    • Putative methyltransferase NSUN5 antibody
    • WBSCR20 antibody
    • WBSCR20A antibody
    • Williams Beuren syndrome chromosome region 20A antibody
    • Williams-Beuren syndrome chromosomal region 20A protein antibody
    • Williams-Beuren syndrome critical region protein 20 copy A antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121633 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-NSUN5 antibody (ab121633) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique.
  • ab121633 at 1/250 dilution staining NSUN5 in Paraffin-embedded Human rectum tissue by Immunohistochemistry.

References

ab121633 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mESCs)
Gel Running Conditions
Non-reduced Denaturing (4-20)
Loading amount
30 µg
Specification
mESCs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

Submitted Jan 06 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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