Overview

  • Product name

  • Description

    Rabbit polyclonal to NSUN6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NSUN6 aa 80-130 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    NGLSVPILQHPDLQDVLLIPVIGPRKNIKKQQCEAIVGAQCGNAVLRGAH V


    Database link: Q8TEA1

  • Positive control

    • Human breast cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May have S-adenosyl-L-methionine-dependent methyl-transferase activity.
  • Sequence similarities

    Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.
    Contains 1 PUA domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4933414E04Rik antibody
    • FLJ23743 antibody
    • NOL1/NOP2/Sun and PUA domain-containing protein 1 antibody
    • NOL1/NOP2/Sun domain family 6 antibody
    • NOL1/NOP2/Sun domain family member 6 antibody
    • NOP2 antibody
    • NOPD1 antibody
    • NSUN6 antibody
    • NSUN6_HUMAN antibody
    • nucleolar protein (NOL1/NOP2/sun) and PUA domains 1 antibody
    • Putative methyltransferase NSUN6 antibody
    • Sun domain family, member 6 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling NSUN6 with ab214227 at 1/500 dilution, followed by secondary antibody detection and DAB staining.

References

This product has been referenced in:

  • Yang XS  et al. Hypoxia-inducible factor-1 alpha is involved in RIP-induced necroptosis caused by in vitro and in vivo ischemic brain injury. Sci Rep 7:5818 (2017). Read more (PubMed: 28724891) »
See 1 Publication for this product

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