Anti-NT-proBNP antibody [15F11] (ab13115)
Key features and details
- Mouse monoclonal [15F11] to NT-proBNP
- Suitable for: ELISA, WB
- Reacts with: Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-NT-proBNP antibody [15F11] -
Description
Mouse monoclonal [15F11] to NT-proBNP -
Host species
Mouse -
Tested Applications & Species
Application Species ELISA Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human NT-proBNP aa 1-100.
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Positive control
- Recombinant Human BNP protein (ab87200) can be used as a positive control in WB.
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General notes
Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Chromatography on protein A Sepharose -
Clonality
Monoclonal -
Clone number
15F11 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ELISA |
Recombinant fragment
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Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 13 kDa.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 13 kDa. |
Target
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Function
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Tissue specificity
Brain and also in atria, but at much lower levels than ANP. -
Sequence similarities
Belongs to the natriuretic peptide family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANFB_HUMAN antibody
- BNP(1-32) antibody
- BNP(5-29) antibody
see all
Images
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WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].
References (2)
ab13115 has been referenced in 2 publications.
- Nagai H et al. Antihypertrophic Effects of Small Molecules that Maintain Mitochondrial ATP Levels Under Hypoxia. EBioMedicine 24:147-158 (2017). PubMed: 28942281
- Tonne JM et al. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Clin Chem 57:864-73 (2011). IP ; Human . PubMed: 21482747