Anti-NT-proBNP antibody [15F11] (ab13115)

Submit a review Q&A (1)References (5)
Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)
  • ELISA - Anti-NT-proBNP antibody [15F11] (ab13115)

Key features and details

  • Mouse monoclonal [15F11] to NT-proBNP
  • Suitable for: ELISA, WB
  • Reacts with: Recombinant fragment
  • Isotype: IgG2b

Overview

  • Product name

    Anti-NT-proBNP antibody [15F11]
    See all NT-proBNP primary antibodies

  • Description

    Mouse monoclonal [15F11] to NT-proBNP

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Recombinant fragment

  • Immunogen

    Synthetic peptide corresponding to Human NT-proBNP aa 1-100.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human BNP protein (ab87200) can be used as a positive control in WB.

  • General notes

    Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab13115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 13 kDa.
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 13 kDa.

Target

  • Function

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • Tissue specificity

    Brain and also in atria, but at much lower levels than ANP.

  • Sequence similarities

    Belongs to the natriuretic peptide family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • ANFB_HUMAN antibody
    • BNP(1-32) antibody
    • BNP(5-29) antibody
    • BNP-32 antibody
    • Gamma-brain natriuretic peptide antibody
    • nppb antibody
    see all

Images

  • Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)
    Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)
    WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].
  • ELISA - Anti-NT-proBNP antibody [15F11] (ab13115)
    ELISA - Anti-NT-proBNP antibody [15F11] (ab13115)
    NT-proBNP calibration curves using pairs ab14703 [10H4] and ab13111 [5B6] (grey), and ab13115 [15F11] and ab13111 [5B6] (black), (capture: 2 µg/well, detection: Eu-labeled 5B6; 200 ng/well). Antigen was recombinant NT-proBNP. Incubated 1 hr at room temperature

References (5)

Publishing research using ab13115? Please let us know so that we can cite the reference in this datasheet.

ab13115 has been referenced in 5 publications.

  • Karhu ST  et al. GATA4-targeted compound exhibits cardioprotective actions against doxorubicin-induced toxicity in vitro and in vivo: establishment of a chronic cardiotoxicity model using human iPSC-derived cardiomyocytes. Arch Toxicol 94:2113-2130 (2020). PubMed: 32185414
  • Pohjolainen L  et al. Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Front Pharmacol 11:553852 (2020). PubMed: 33584253
  • Mosqueira D  et al. High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy In Vitro. Methods Protoc 2:N/A (2019). PubMed: 31717790
  • Nagai H  et al. Antihypertrophic Effects of Small Molecules that Maintain Mitochondrial ATP Levels Under Hypoxia. EBioMedicine 24:147-158 (2017). PubMed: 28942281
  • Tonne JM  et al. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Clin Chem 57:864-73 (2011). IP ; Human . PubMed: 21482747

Customer reviews and Q&As

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Question

Inquiry: Dear Sir/Madam, I am looking for antibodies directed at or cross-reactive with rat NT-pro-BNP. Preferably monoclonal and source from any species. There are multiple monoclonal available on your site, but directed to human. Do you have antibodies that are crossreactive to rat. Best Regards,

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Answer

Thank you very much for your inquiry.

I have to confirm that do not have any pro-BNP antibodies that are tested and guaranteed for rat.

I checked the immunogens for the available pro-BNP antibodies and unfortunately I cannot recommend any of for testing in rat.

We usually expect an cross-reaction when the homology is 85% and higher. But the different regions that the immunogens are taken from only show 62% and less homology:

AA 1-12 no homology
AA 13-27 58%
AA 28-45 62%
AA 61-76 no homology.

I am sorry I could not be of more help on thisoccasion and wish you goo dluck with your research.

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