Anti-NT2NL antibody (ab233287)
Key features and details
- Rabbit polyclonal to NT2NL
- Suitable for: IHC-P, WB
- Reacts with: Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-NT2NL antibody -
Description
Rabbit polyclonal to NT2NL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Cow, Human -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human NT2NL aa 1 to the C-terminus. Expressed in E.coli. N-terminal tags.
Database link: Q7Z3S9 -
Positive control
- IHC-P: Human stomach tissue. WB: Recombinant human NT2NL protein; Human lung lysate; HeLa and A549 lysate; Bovine testis lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Function
May function in the Notch signaling pathway and regulate neutrophil differentiation. -
Tissue specificity
Widely expressed with higher levels in leukocytes and lymph nodes. -
Sequence similarities
Belongs to the NOTCH family.
Contains 6 EGF-like domains. -
Post-translational
modificationsCleaved by ELANE. -
Cellular localization
Secreted. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 388677 Human
- SwissProt: Q7Z3S9 Human
- Unigene: 655156 Human
- Unigene: 683989 Human
- Unigene: 728902 Human
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Alternative names
- N2N antibody
- Notch 2 N terminal like antibody
- Notch homolog 2 (Drosophila) N-terminal like antibody
see all
Images
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All lanes : Anti-NT2NL antibody (ab233287) at 1 µg/ml
Lane 1 : Human lung tissue lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : Bovine testis tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution -
Formalin-fixed, paraffin-embedded human stomach tissue stained for NT2NL using ab233287 at 10 µg/mL in immunohistochemical analysis. DAB staining.
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Anti-NT2NL antibody (ab233287) at 2 µg/ml + Recombinant human NT2NL protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab233287 has been referenced in 2 publications.
- Yu J et al. GGC repeat expansions in NOTCH2NLC causing a phenotype of distal motor neuropathy and myopathy. Ann Clin Transl Neurol 8:1330-1342 (2021). PubMed: 33943039
- Yu J et al. The GGC repeat expansion in NOTCH2NLC is associated with oculopharyngodistal myopathy type 3. Brain 144:1819-1832 (2021). PubMed: 33693509