Key features and details
- Rabbit polyclonal to NT5C1A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NT5C1A antibody
See all NT5C1A primary antibodies
DescriptionRabbit polyclonal to NT5C1A
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- Human skeletal muscle tissue; A549 cells; HepG2 and MCF7 cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/1000. Predicted molecular weight: 41 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionDephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.
Tissue specificityHighly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas.
Sequence similaritiesBelongs to the 5'-nucleotidase type 3 family.
- Information by UniProt
- 5' nucleotidase cytosolic IA antibody
- 5NT1A_HUMAN antibody
- AMP specific 5' NT antibody
Immunohistochemical analysis of formalin fixed, paraffin embedded Human skeletal muscle tissue labeling NT5C1A using ab190214 at 10 µg/ml.
Immunofluorescent analysis of A549 cells labeling NT5C1A using ab190214 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
All lanes : Anti-NT5C1A antibody (ab190214) at 1/500 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : MCF7 cell lysate treated with the synthesized peptide
Predicted band size: 41 kDa
ab190214 has not yet been referenced specifically in any publications.