Anti-NTAL antibody [NAP-07] (ab3992)

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Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)

    Key features and details

    • Mouse monoclonal [NAP-07] to NTAL
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    PE

    Overview

    • Product name

      Anti-NTAL antibody [NAP-07]
      See all NTAL primary antibodies

    • Description

      Mouse monoclonal [NAP-07] to NTAL

    • Host species

      Mouse

    • Specificity

      The antibody reacts with Non-T cell activation linker (NTAL) a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL.

    • Positive control

      • RAMOS human lymphoma cell lysate, Raji human lymphoma cell lysate

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3992 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.

    Target

    • Function

      Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.

    • Tissue specificity

      Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).

    • Involvement in disease

      Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

    • Post-translational
      modifications

      Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
      May be polyubiquitinated.

    • Cellular localization

      Cell membrane. Present in lipid rafts.
    • Information by UniProt

    • Database links

      • Alternative names

        • HSPC046 antibody
        • LAB antibody
        • LAT2 antibody
        • Linker for activation of B cells antibody
        • Linker for activation of B-cells antibody
        • Linker for activation of T cells family member 2 antibody
        • Linker for activation of T cells transmembrane adaptor 2 antibody
        • Linker for activation of T-cells family member 2 antibody
        • Membrane associated adapter molecule antibody
        • Membrane-associated adapter molecule antibody
        • Non T cell activation linker antibody
        • Non-T-cell activation linker antibody
        • NTAL_HUMAN antibody
        • WBSCR 5 antibody
        • Wbscr15 antibody
        • Williams Beuren syndrome chromosome region 15 antibody
        • Williams Beuren syndrome chromosome region 5 antibody
        • Williams-Beuren syndrome chromosomal region 15 protein antibody
        • Williams-Beuren syndrome chromosomal region 5 protein antibody
        • WSCR5 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)
        Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)
        Subcellular localization of NTAL by confocal microscopy in the myeloid cell line THP-1. THP-1 cells were permeabilized and immunostained using antibody NAP-07 (green). Nuclei are vizualized by propidium iodide (red).

      References (8)

      Publishing research using ab3992? Please let us know so that we can cite the reference in this datasheet.

      ab3992 has been referenced in 8 publications.

      • Malhotra S  et al. Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB. J Biol Chem 284:36202-12 (2009). WB ; Mouse . PubMed: 19858206
      • Mutch CM  et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). PubMed: 17090619
      • Macaulay IC  et al. Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins. Blood 109:3260-9 (2007). PubMed: 17192395
      • Dráberová L  et al. Regulation of Ca2+ signaling in mast cells by tyrosine-phosphorylated and unphosphorylated non-T cell activation linker. J Immunol 179:5169-80 (2007). PubMed: 17911602
      • Lebduska P  et al. Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods 328:139-51 (2007). PubMed: 17900607
      • Heneberg P  et al. Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol 36:2795-806 (2006). PubMed: 17013982
      • Volná P  et al. Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med 200:1001-13 (2004). PubMed: 15477348
      • Tkaczyk C  et al. NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood 104:207-14 (2004). PubMed: 15010370

      Customer reviews and Q&As

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      Western blot abreview for Anti-NTAL antibody [NAP-07]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - other (RAW264.7)
      Loading amount
      40 µg
      Specification
      RAW264.7
      Gel Running Conditions
      Non-reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

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      Verified customer

      Submitted Oct 24 2007

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