For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    ntal-antibody-nap-07-ab3992.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email

Anti-NTAL antibody [NAP-07] (ab3992)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (8)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)

    Key features and details

    • Mouse monoclonal [NAP-07] to NTAL
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    PE

    You may also be interested in

    Primary
    Product image
    Anti-CD13 antibody [SP182] (ab183358)
    Primary
    Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204)
    Primary
    Product image
    PE Anti-NTAL antibody [NAP-07] (ab27574)

    View more associated products

    Overview

    • Product name

      Anti-NTAL antibody [NAP-07]
      See all NTAL primary antibodies
    • Description

      Mouse monoclonal [NAP-07] to NTAL
    • Host species

      Mouse
    • Specificity

      The antibody reacts with Non-T cell activation linker (NTAL) a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL.

    • Positive control

      • RAMOS human lymphoma cell lysate, Raji human lymphoma cell lysate
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
    • Clonality

      Monoclonal
    • Clone number

      NAP-07
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Adaptive Immunity
      • T Cells
      • Non-CD
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Other

    Associated products

    • Alternative Versions

      • PE Anti-NTAL antibody [NAP-07] (ab27574)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Mouse NTAL protein (ab56522)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3992 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.

    Target

    • Function

      Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.
    • Tissue specificity

      Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).
    • Involvement in disease

      Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Post-translational
      modifications

      Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
      May be polyubiquitinated.
    • Cellular localization

      Cell membrane. Present in lipid rafts.
    • Target information above from: UniProt accession Q9GZY6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7462 Human
      • Omim: 605719 Human
      • SwissProt: Q9GZY6 Human
      • Unigene: 726075 Human
      • Alternative names

        • HSPC046 antibody
        • LAB antibody
        • LAT2 antibody
        • Linker for activation of B cells antibody
        • Linker for activation of B-cells antibody
        • Linker for activation of T cells family member 2 antibody
        • Linker for activation of T cells transmembrane adaptor 2 antibody
        • Linker for activation of T-cells family member 2 antibody
        • Membrane associated adapter molecule antibody
        • Membrane-associated adapter molecule antibody
        • Non T cell activation linker antibody
        • Non-T-cell activation linker antibody
        • NTAL_HUMAN antibody
        • WBSCR 5 antibody
        • Wbscr15 antibody
        • Williams Beuren syndrome chromosome region 15 antibody
        • Williams Beuren syndrome chromosome region 5 antibody
        • Williams-Beuren syndrome chromosomal region 15 protein antibody
        • Williams-Beuren syndrome chromosomal region 5 protein antibody
        • WSCR5 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)
        Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)
        Subcellular localization of NTAL by confocal microscopy in the myeloid cell line THP-1. THP-1 cells were permeabilized and immunostained using antibody NAP-07 (green). Nuclei are vizualized by propidium iodide (red).

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (8)

      Publishing research using ab3992? Please let us know so that we can cite the reference in this datasheet.

      ab3992 has been referenced in 8 publications.

      • Malhotra S  et al. Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB. J Biol Chem 284:36202-12 (2009). WB ; Mouse . PubMed: 19858206
      • Mutch CM  et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). PubMed: 17090619
      • Macaulay IC  et al. Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins. Blood 109:3260-9 (2007). PubMed: 17192395
      • Dráberová L  et al. Regulation of Ca2+ signaling in mast cells by tyrosine-phosphorylated and unphosphorylated non-T cell activation linker. J Immunol 179:5169-80 (2007). PubMed: 17911602
      • Lebduska P  et al. Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods 328:139-51 (2007). PubMed: 17900607
      • Heneberg P  et al. Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol 36:2795-806 (2006). PubMed: 17013982
      • Volná P  et al. Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med 200:1001-13 (2004). PubMed: 15477348
      • Tkaczyk C  et al. NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood 104:207-14 (2004). PubMed: 15010370

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-NTAL antibody [NAP-07]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - other (RAW264.7)
      Loading amount
      40 µg
      Specification
      RAW264.7
      Gel Running Conditions
      Non-reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 24 2007

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2022 Abcam plc. All rights reserved.