Anti-NTAL antibody [NAP-07] (ab3992)
Key features and details
- Mouse monoclonal [NAP-07] to NTAL
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-NTAL antibody [NAP-07]
See all NTAL primary antibodies -
Description
Mouse monoclonal [NAP-07] to NTAL -
Host species
Mouse -
Specificity
The antibody reacts with Non-T cell activation linker (NTAL) a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells. -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL.
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Positive control
- RAMOS human lymphoma cell lysate, Raji human lymphoma cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
NAP-07 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab3992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Use under non reducing condition. (see Tkaczyk et al). | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent dilution. |
Target
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Function
Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2. -
Tissue specificity
Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level). -
Involvement in disease
Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
Post-translational
modificationsPhosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
May be polyubiquitinated. -
Cellular localization
Cell membrane. Present in lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 7462 Human
- Entrez Gene: 56743 Mouse
- Omim: 605719 Human
- SwissProt: Q9GZY6 Human
- SwissProt: Q9JHL0 Mouse
- Unigene: 726075 Human
- Unigene: 391375 Mouse
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Alternative names
- HSPC046 antibody
- LAB antibody
- LAT2 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)](https://www.abcam.com/ps/products/3/ab3992/Images/ab3992_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)Subcellular localization of NTAL by confocal microscopy in the myeloid cell line THP-1. THP-1 cells were permeabilized and immunostained using antibody NAP-07 (green). Nuclei are vizualized by propidium iodide (red).
Protocols
References
ab3992 has been referenced in 8 publications.
- Malhotra S et al. Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB. J Biol Chem 284:36202-12 (2009). WB ; Mouse . PubMed: 19858206
- Mutch CM et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). PubMed: 17090619
- Macaulay IC et al. Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins. Blood 109:3260-9 (2007). PubMed: 17192395
- Dráberová L et al. Regulation of Ca2+ signaling in mast cells by tyrosine-phosphorylated and unphosphorylated non-T cell activation linker. J Immunol 179:5169-80 (2007). PubMed: 17911602
- Lebduska P et al. Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods 328:139-51 (2007). PubMed: 17900607
- Heneberg P et al. Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol 36:2795-806 (2006). PubMed: 17013982
- Volná P et al. Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med 200:1001-13 (2004). PubMed: 15477348
- Tkaczyk C et al. NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood 104:207-14 (2004). PubMed: 15010370
