Anti-NTAL antibody [NAP-07] (ab3992)

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Overview

  • Product name
    Anti-NTAL antibody [NAP-07]
    See all NTAL primary antibodies
  • Description
    Mouse monoclonal [NAP-07] to NTAL
  • Host species
    Mouse
  • Specificity
    The antibody reacts with Non-T cell activation linker (NTAL) a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL.

  • Positive control
    • RAMOS human lymphoma cell lysate, Raji human lymphoma cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab3992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Use under non reducing condition. (see Tkaczyk et al).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.
  • Tissue specificity
    Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).
  • Involvement in disease
    Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Post-translational
    modifications
    Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
    May be polyubiquitinated.
  • Cellular localization
    Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • Database links
    • Alternative names
      • HSPC046 antibody
      • LAB antibody
      • LAT2 antibody
      • Linker for activation of B cells antibody
      • Linker for activation of B-cells antibody
      • Linker for activation of T cells family member 2 antibody
      • Linker for activation of T cells transmembrane adaptor 2 antibody
      • Linker for activation of T-cells family member 2 antibody
      • Membrane associated adapter molecule antibody
      • Membrane-associated adapter molecule antibody
      • Non T cell activation linker antibody
      • Non-T-cell activation linker antibody
      • NTAL_HUMAN antibody
      • WBSCR 5 antibody
      • Wbscr15 antibody
      • Williams Beuren syndrome chromosome region 15 antibody
      • Williams Beuren syndrome chromosome region 5 antibody
      • Williams-Beuren syndrome chromosomal region 15 protein antibody
      • Williams-Beuren syndrome chromosomal region 5 protein antibody
      • WSCR5 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)
      Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)
      Subcellular localization of NTAL by confocal microscopy in the myeloid cell line THP-1. THP-1 cells were permeabilized and immunostained using antibody NAP-07 (green). Nuclei are vizualized by propidium iodide (red).

    References

    This product has been referenced in:
    • Malhotra S  et al. Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB. J Biol Chem 284:36202-12 (2009). WB ; Mouse . Read more (PubMed: 19858206) »
    • Mutch CM  et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). Read more (PubMed: 17090619) »
    See all 8 Publications for this product

    Publishing research using ab3992? Please let us know so that we can cite the reference in this datasheet.

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    Western blot abreview for Anti-NTAL antibody [NAP-07]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (RAW264.7)
    Loading amount
    40 µg
    Specification
    RAW264.7
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

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    Verified customer

    Submitted Oct 24 2007

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