Overview

  • Product name

    Anti-NTE antibody - C-terminal
  • Description

    Rabbit polyclonal to NTE - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Recombinant fragment within Human NTE (C terminal). The exact sequence is proprietary.
    Database link: Q8IY17

  • Positive control

    • IHC-P: Mouse hindbrain tissue; rat forebrain tissue. WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts; rat brain tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab228683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 150 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy.
  • Tissue specificity

    Expressed in brain, placenta, kidney, neuron and skeletal muscle.
  • Involvement in disease

    Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39) [MIM:612020]; also known as NTE-related motor neuron disorder (NTEMND). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG39 is associated with a motor axonopathy affecting upper and lower limbs and resulting in progressive wasting of distal upper and lower extremity muscles.
  • Sequence similarities

    Belongs to the NTE family.
    Contains 3 cyclic nucleotide-binding domains.
    Contains 1 patatin domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane. Anchored to the cytoplasmic face of the endoplasmic reticulum by its amino-terminal transmembrane segment.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 3.1.1.5 antibody
    • Neuropathy target esterase antibody
    • NTEMND antibody
    • Patatin like phospholipase domain containing 6 antibody
    • Patatin like phospholipase domain containing protein 6 antibody
    • Patatin-like phospholipase domain-containing protein 6 antibody
    • PLPL6_HUMAN antibody
    • Pnpla6 antibody
    • SPG39 antibody
    • sws antibody
    see all

Images

  • Paraffin-embedded mouse hindbrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extracts
    Lane 3 : IMR32 (human neuroblast cell line) whole cell extracts
    Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 150 kDa



    5% SDS-PAGE

  • Paraffin-embedded rat forebrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.

  • Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution + Rat brain tissue extract at 50 µg

    Developed using the ECL technique.

    Predicted band size: 150 kDa



    5% SDS-PAGE

References

ab228683 has not yet been referenced specifically in any publications.

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