Anti-NTE antibody - C-terminal (ab228683)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NTE antibody - C-terminal -
Description
Rabbit polyclonal to NTE - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow -
Immunogen
Recombinant fragment within Human NTE (C terminal). The exact sequence is proprietary.
Database link: Q8IY17 -
Positive control
- IHC-P: Mouse hindbrain tissue; rat forebrain tissue. WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts; rat brain tissue extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab228683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/500 - 1/3000. Predicted molecular weight: 150 kDa. | |
IHC-P | 1/100 - 1/1000. |
Target
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Function
Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. -
Tissue specificity
Expressed in brain, placenta, kidney, neuron and skeletal muscle. -
Involvement in disease
Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39) [MIM:612020]; also known as NTE-related motor neuron disorder (NTEMND). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG39 is associated with a motor axonopathy affecting upper and lower limbs and resulting in progressive wasting of distal upper and lower extremity muscles. -
Sequence similarities
Belongs to the NTE family.
Contains 3 cyclic nucleotide-binding domains.
Contains 1 patatin domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Endoplasmic reticulum membrane. Anchored to the cytoplasmic face of the endoplasmic reticulum by its amino-terminal transmembrane segment. - Information by UniProt
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Database links
- Entrez Gene: 425396 Chicken
- Entrez Gene: 767822 Cow
- Entrez Gene: 10908 Human
- Entrez Gene: 50767 Mouse
- Entrez Gene: 360753 Rat
- Omim: 603197 Human
- SwissProt: Q8IY17 Human
- SwissProt: Q3TRM4 Mouse
see all -
Alternative names
- EC 3.1.1.5 antibody
- Neuropathy target esterase antibody
- NTEMND antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683)
Paraffin-embedded mouse hindbrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extracts
Lane 3 : IMR32 (human neuroblast cell line) whole cell extracts
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 150 kDa5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683)
Paraffin-embedded rat forebrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.
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Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 150 kDa5% SDS-PAGE
References
ab228683 has not yet been referenced specifically in any publications.