Anti-NTE antibody - C-terminal (ab228683)

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Key features and details

  • Rabbit polyclonal to NTE - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-NTE antibody - C-terminal

  • Description

    Rabbit polyclonal to NTE - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow

  • Immunogen

    Recombinant fragment within Human NTE (C terminal). The exact sequence is proprietary.
    Database link: Q8IY17

  • Positive control

    • IHC-P: Mouse hindbrain tissue; rat forebrain tissue. WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts; rat brain tissue extract.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 150 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy.

  • Tissue specificity

    Expressed in brain, placenta, kidney, neuron and skeletal muscle.

  • Involvement in disease

    Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39) [MIM:612020]; also known as NTE-related motor neuron disorder (NTEMND). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG39 is associated with a motor axonopathy affecting upper and lower limbs and resulting in progressive wasting of distal upper and lower extremity muscles.

  • Sequence similarities

    Belongs to the NTE family.
    Contains 3 cyclic nucleotide-binding domains.
    Contains 1 patatin domain.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Endoplasmic reticulum membrane. Anchored to the cytoplasmic face of the endoplasmic reticulum by its amino-terminal transmembrane segment.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EC 3.1.1.5 antibody
    • Neuropathy target esterase antibody
    • NTEMND antibody
    • Patatin like phospholipase domain containing 6 antibody
    • Patatin like phospholipase domain containing protein 6 antibody
    • Patatin-like phospholipase domain-containing protein 6 antibody
    • PLPL6_HUMAN antibody
    • Pnpla6 antibody
    • SPG39 antibody
    • sws antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683)

    Paraffin-embedded mouse hindbrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-NTE antibody - C-terminal (ab228683)
    Western blot - Anti-NTE antibody - C-terminal (ab228683)
    All lanes : Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extracts
    Lane 3 : IMR32 (human neuroblast cell line) whole cell extracts
    Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 150 kDa



    5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTE antibody - C-terminal (ab228683)

    Paraffin-embedded rat forebrain tissue stained for NTE with ab228683 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-NTE antibody - C-terminal (ab228683)
    Western blot - Anti-NTE antibody - C-terminal (ab228683)
    Anti-NTE antibody - C-terminal (ab228683) at 1/1000 dilution + Rat brain tissue extract at 50 µg

    Developed using the ECL technique.

    Predicted band size: 150 kDa



    5% SDS-PAGE

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab228683? Please let us know so that we can cite the reference in this datasheet.

ab228683 has not yet been referenced specifically in any publications.

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